Silence of circPLK1 inhibits NSCLC progression by regulating the miR-1294/SLC16A9 axis.
[BACKGROUND] Non-small cell lung cancer (NSCLC) is the malignancy with the highest mortality worldwide.
APA
Li C, Yang Y, et al. (2026). Silence of circPLK1 inhibits NSCLC progression by regulating the miR-1294/SLC16A9 axis.. Molecular immunology, 189, 216-225. https://doi.org/10.1016/j.molimm.2025.11.020
MLA
Li C, et al.. "Silence of circPLK1 inhibits NSCLC progression by regulating the miR-1294/SLC16A9 axis.." Molecular immunology, vol. 189, 2026, pp. 216-225.
PMID
41443149
Abstract
[BACKGROUND] Non-small cell lung cancer (NSCLC) is the malignancy with the highest mortality worldwide. Circular RNA polo-like kinase 1 (circPLK1) is a circular RNA that involved in cancer progression. However, the role of circPLK1 in NSCLC requires further investigation.
[METHODS] The expression of circPLK1 in NSCLC cells was measured by using qRT-PCR. Bioinformatics analysis was performed to identify miRNAs that bind to circPLK1 and the target mRNAs of these miRNAs. Cell viability, proliferation, apoptosis, migration, and invasion were assessed using CCK-8, colony formation, flow cytometry, and Transwell assays, respectively. Protein expression was evaluated by western blotting. Xenograft models were established to verify the regulatory role of circPLK1 in the miR-1294/SLC16A9 axis.
[RESULTS] circPLK1 was upregulaterd in NSCLC cells. Silencing circPLK1 markedly inhibited cell viability, proliferation, migration, and invasion, while accelerating apoptosis in NSCLC cells. circPLK1 acted as a sponge for miR-1294 and promoted SLC16A9 expression in NSCLC cells. Knockdown of circPLK1 suppressed tumor growth in NSCLC by regulating the miR-1294/SLC16A9 axis.
[CONCLUSION] circPLK1 silence inhibited the proliferation, migration, and invasion of NSCLC cells by regulating the miR-1294/SLC16A9 axis.
[METHODS] The expression of circPLK1 in NSCLC cells was measured by using qRT-PCR. Bioinformatics analysis was performed to identify miRNAs that bind to circPLK1 and the target mRNAs of these miRNAs. Cell viability, proliferation, apoptosis, migration, and invasion were assessed using CCK-8, colony formation, flow cytometry, and Transwell assays, respectively. Protein expression was evaluated by western blotting. Xenograft models were established to verify the regulatory role of circPLK1 in the miR-1294/SLC16A9 axis.
[RESULTS] circPLK1 was upregulaterd in NSCLC cells. Silencing circPLK1 markedly inhibited cell viability, proliferation, migration, and invasion, while accelerating apoptosis in NSCLC cells. circPLK1 acted as a sponge for miR-1294 and promoted SLC16A9 expression in NSCLC cells. Knockdown of circPLK1 suppressed tumor growth in NSCLC by regulating the miR-1294/SLC16A9 axis.
[CONCLUSION] circPLK1 silence inhibited the proliferation, migration, and invasion of NSCLC cells by regulating the miR-1294/SLC16A9 axis.
MeSH Terms
Humans; MicroRNAs; Carcinoma, Non-Small-Cell Lung; Lung Neoplasms; RNA, Circular; Cell Proliferation; Animals; Gene Expression Regulation, Neoplastic; Polo-Like Kinase 1; Cell Movement; Cell Line, Tumor; Protein Serine-Threonine Kinases; Mice; Apoptosis; Disease Progression; Mice, Nude; Mice, Inbred BALB C; Cell Survival; Cell Cycle Proteins; Gene Silencing; A549 Cells
같은 제1저자의 인용 많은 논문 (5)
- Combination therapy for colorectal cancer with anti-PD-L1 and cancer vaccine: A multiscale mathematical model of tumor-immune interactions.
- Concurrent MLL-AF4 infant ALL in monozygotic twins: a case report.
- Adaptive and migration-enhanced tree seed algorithm for multi-threshold CT image segmentation and lung cancer recognition.
- Phase II study of olaparib and durvalumab in patients with metastatic castration-resistant prostate cancer.
- Interaction Effects Between Tongue-Rolling Behavior and Chronic Stress on Plasma Immune-Inflammatory Indicators, Milk Protein Composition, and Milk Proteome in Dairy Cows.