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Single-cell spatial transcriptomics reveals tumor microenvironment heterogeneity in primary and lymph node-metastatic small cell lung cancer.

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Cell reports. Medicine 📖 저널 OA 99.2% 2021: 1/1 OA 2024: 9/9 OA 2025: 45/46 OA 2026: 73/73 OA 2021~2026 2026 Vol.7(4) p. 102713 OA Single-cell and spatial transcriptom
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PubMed DOI OpenAlex 마지막 보강 2026-04-29
OpenAlex 토픽 · Single-cell and spatial transcriptomics Cancer Immunotherapy and Biomarkers Ferroptosis and cancer prognosis

Zhang Z, Wu D, Chen R, Zhai M, Yang F, Wang J, Guo L, Liu L, Ying J, Yang L, Zhou M

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Lymph node metastasis (LNM) is a critical prognostic and therapeutic determinant in small cell lung cancer (SCLC), yet its spatial cellular ecosystem remains poorly understood.

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APA Zheng Zhang, Dongfang Wu, et al. (2026). Single-cell spatial transcriptomics reveals tumor microenvironment heterogeneity in primary and lymph node-metastatic small cell lung cancer.. Cell reports. Medicine, 7(4), 102713. https://doi.org/10.1016/j.xcrm.2026.102713
MLA Zheng Zhang, et al.. "Single-cell spatial transcriptomics reveals tumor microenvironment heterogeneity in primary and lymph node-metastatic small cell lung cancer.." Cell reports. Medicine, vol. 7, no. 4, 2026, pp. 102713.
PMID 41916294 ↗

Abstract

Lymph node metastasis (LNM) is a critical prognostic and therapeutic determinant in small cell lung cancer (SCLC), yet its spatial cellular ecosystem remains poorly understood. Here, we perform single-cell spatial transcriptomics using the CosMx Spatial Molecular Imager on 105 primary and metastatic lymph node specimens from 75 SCLC patients, generating a comprehensive atlas of over 600,000 cells. We identify three LNM-enriched malignant subclusters with distinct metabolic and angiogenic programs that spatially correlate with immune exclusion features. Spatial analysis reveals vascular-immune crosstalk, wherein endothelial cells orchestrate immune activation through avoidance of malignant cells while forming functional perivascular niches with cytotoxic T cells during LNM. Cellular neighborhood analysis delineates distinct multicellular niches and identifies a pan-immune hotspot (PIHs-1) whose abundance is an independent predictor of survival. This study provides a high-resolution spatial map of the SCLC tumor microenvironment during LNM and establishes spatially defined architectures as both mechanistic insights and translatable biomarkers.

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