Modeling Breast Tumor Development with a Humanized Mouse Model.
Abstract
The tumor microenvironment plays a critical role in breast cancer growth and progression to metastasis. Here, we describe a method to examine stromal-epithelial interactions during tumor formation and progression utilizing human-derived mammary epithelial cells and breast stromal cells. This method outlines the isolation of each cell type from reduction mammoplasty tissue, the culture and genetic modification of both epithelial and stromal cells using lentiviral technology, and the method of humanizing and implantation of transformed epithelial cells into the cleared mammary fat pads of immunocompromised mice. This model system may be a useful tool to dissect signaling interactions that contribute to invasive tumor behavior and therapeutic resistance.
추출된 의학 개체 (NER)
| 유형 | 영어 표현 | 한국어 / 풀이 | UMLS CUI | 출처 | 등장 |
|---|---|---|---|---|---|
| 해부 | breast
|
유방 | dict | 3 | |
| 해부 | mammary
|
유방 | dict | 2 | |
| 시술 | reduction mammoplasty
|
유방성형술 | dict | 1 | |
| 해부 | stromal-epithelial
|
scispacy | 1 | ||
| 해부 | human-derived mammary epithelial cells
|
scispacy | 1 | ||
| 해부 | breast stromal cells
|
scispacy | 1 | ||
| 해부 | cell
|
scispacy | 1 | ||
| 해부 | tissue
|
scispacy | 1 | ||
| 해부 | epithelial
|
scispacy | 1 | ||
| 해부 | stromal cells
|
scispacy | 1 | ||
| 해부 | epithelial cells
|
scispacy | 1 | ||
| 해부 | mammary fat pads
|
scispacy | 1 | ||
| 질환 | Breast Tumor
|
C1458155
Mammary Neoplasms
|
scispacy | 1 | |
| 질환 | tumor
|
C0027651
Neoplasms
|
scispacy | 1 | |
| 질환 | breast cancer
|
C0006142
Malignant neoplasm of breast
|
scispacy | 1 | |
| 기타 | Mouse
|
scispacy | 1 | ||
| 기타 | lentiviral
|
scispacy | 1 | ||
| 기타 | mice
|
scispacy | 1 |
MeSH Terms
Animals; Cell Communication; Disease Models, Animal; Epithelial Cells; Female; Heterografts; Humans; Mammary Neoplasms, Animal; Mice; Stromal Cells; Tumor Microenvironment
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