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Evaluation of New Coumarins for Anti-Cancer Activity in HL-60 Cell Line Supported by Molecular Docking, MD Simulation, and Binding Free Energy Calculations.

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OncoTargets and therapy 📖 저널 OA 100% 2024: 7/7 OA 2025: 22/22 OA 2026: 22/22 OA 2024~2026 2026 Vol.19() p. 554081 OA
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Humayun N, Rabbi F, Sohail M, Khan A, Zeb A, Nisar A

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[INTRODUCTION] Leukemia, a prevalent malignancy characterized by chromosomal aberrations, was targeted in this study to assess the anti-cancer potential of naturally isolated novel coumarins.

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APA Humayun N, Rabbi F, et al. (2026). Evaluation of New Coumarins for Anti-Cancer Activity in HL-60 Cell Line Supported by Molecular Docking, MD Simulation, and Binding Free Energy Calculations.. OncoTargets and therapy, 19, 554081. https://doi.org/10.2147/OTT.S554081
MLA Humayun N, et al.. "Evaluation of New Coumarins for Anti-Cancer Activity in HL-60 Cell Line Supported by Molecular Docking, MD Simulation, and Binding Free Energy Calculations.." OncoTargets and therapy, vol. 19, 2026, pp. 554081.
PMID 41908095 ↗
DOI 10.2147/OTT.S554081

Abstract

[INTRODUCTION] Leukemia, a prevalent malignancy characterized by chromosomal aberrations, was targeted in this study to assess the anti-cancer potential of naturally isolated novel coumarins.

[METHODS] Novel coumarins (isolated from the natural source ) were assessed against HL-60 leukemia cells using in vitro and in silico approaches.

[RESULTS] The MTT assay demonstrated significant cytotoxicity of the compounds against HL-60 cells. Molecular docking studies revealed strong binding interactions with CDK-2 and BCL-2 proteins, supporting the in vitro results. Compound 2 exhibited the highest binding energy with CDK-2. Molecular dynamics simulations, binding free energy calculations, principal component analysis (PCA), and free energy landscape analyses were performed to assess the stability of the Compound 2-CDK-2 complex. The results indicated that the complex remained stable during the MD simulation with favorable binding free energies. ADMET predictions confirmed favorable pharmacokinetic profiles and safety from hERG blockade.

[CONCLUSION] These findings highlight the compounds' promising pharmacological activity and potential for further drug development, though additional studies are needed to explore their clinical applications.

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