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Protocol for the creation and utilization of 3D pancreatic cancer models from circulating tumor cells.

STAR protocols 2025 Vol.6(1) p. 103635

Tang J, Zheng Q, Wang Q, Zhao Y, Ananthanarayanan P, Reina C, Šabanović B, Jiang K, Yang MH, Meny CC, Wang H, Agerbaek MØ, Clausen TM, Gustavsson T, Wen C, Borghi F, Mellano A, Fenocchio E, Gregorc V, Sapino A, Theander TG, Fu D, Aicher A, Salanti A, Shen B, Heeschen C

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We introduce a protocol for generating 3D organoids from circulating tumor cells (CTCs), enabling longitudinal functional and molecular analyses in pancreatic cancer patients, including those with unr

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APA Tang J, Zheng Q, et al. (2025). Protocol for the creation and utilization of 3D pancreatic cancer models from circulating tumor cells.. STAR protocols, 6(1), 103635. https://doi.org/10.1016/j.xpro.2025.103635
MLA Tang J, et al.. "Protocol for the creation and utilization of 3D pancreatic cancer models from circulating tumor cells.." STAR protocols, vol. 6, no. 1, 2025, pp. 103635.
PMID 39946239
DOI 10.1016/j.xpro.2025.103635

Abstract

We introduce a protocol for generating 3D organoids from circulating tumor cells (CTCs), enabling longitudinal functional and molecular analyses in pancreatic cancer patients, including those with unresectable disease, which constitutes the majority of cases. We outline the process for isolating and characterizing CTCs from the blood of pancreatic cancer patients and provide detailed instructions for initiating, passaging, and phenotyping CTC-derived organoids. Additionally, we describe techniques for utilizing these organoids in drug screening with a focus on stemness-related pathways. For complete details on the use and execution of this protocol, please refer to Tang et al..

MeSH Terms

Humans; Neoplastic Cells, Circulating; Pancreatic Neoplasms; Organoids; Cell Culture Techniques, Three Dimensional; Cell Line, Tumor

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