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A cell-state axis underlying colonization in carcinomas with implications for metastasis risk prediction and interception.

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Cell reports 📖 저널 OA 53.3% 2022: 1/1 OA 2024: 6/12 OA 2025: 20/55 OA 2026: 38/54 OA 2022~2026 2025 Vol.44(12) p. 116701
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Handler JS, Li Z, Dveirin RK, Lin JD, Fang W, Wu S

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Metastasis to the liver drives mortality in pancreatic ductal adenocarcinoma (PDAC), yet mechanisms of colonization remain unclear.

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APA Handler JS, Li Z, et al. (2025). A cell-state axis underlying colonization in carcinomas with implications for metastasis risk prediction and interception.. Cell reports, 44(12), 116701. https://doi.org/10.1016/j.celrep.2025.116701
MLA Handler JS, et al.. "A cell-state axis underlying colonization in carcinomas with implications for metastasis risk prediction and interception.." Cell reports, vol. 44, no. 12, 2025, pp. 116701.
PMID 41401069 ↗

Abstract

Metastasis to the liver drives mortality in pancreatic ductal adenocarcinoma (PDAC), yet mechanisms of colonization remain unclear. Using genomic barcoding, we developed a clonal competition model under immune surveillance, isolating murine PDAC subclones with high or low liver-colonization potential. Combined transcriptome and chromatin-accessibility analyses revealed a distinct "metastatic-potential axis," separate from the normal-to-PDAC and classical-basal axes. We established "MetScore" as a biomarker of this axis. MetScore distinguishes metastases from primary PDAC tumors in patients, predicts outcomes beyond classical-basal classifications, and generalizes across carcinoma subtypes, suggesting conserved colonization mechanisms. High-MetScore PDAC cells preferentially occupy immune cell-enriched niches, suggesting they remodel the metastatic microenvironment. Functional screening identified c-Fos as a positive mediator of colonization and a candidate anti-metastatic target. Collectively, we identify a cell-state axis underpinning PDAC liver colonization, introduce MetScore as a broadly applicable biomarker, and nominate actionable targets for peri-operative therapeutic intervention.

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