LINC00963 Targets miR-495-3p to Regulate Pancreatic Cancer Cell Proliferation, Apoptosis, and Carboplatin Sensitivity.

[BACKGROUND AND AIM] Pancreatic cancer (PC) remains one of the most aggressive malignancies worldwide, characterized by rapid disease progression and a dismal prognosis. Despite therapeutic advancements, the development of chemoresistance, particularly to platinum-based agents such as carboplatin, poses a major obstacle to effective clinical management. LINC00963 has been implicated in cancer cell proliferation and drug resistance, although its specific function and underlying mechanisms in PC remain insufficiently explored. The purpose of this paper is to analyze the impact ofLINC00963 on the malignant progression of PC and carboplatin sensibility and the underlying mechanism.

[OBJECTIVE] The purpose of this paper is to enquire the biologic impact of long intergenic non-coding RNA 00963 (LINC00963) in pancreatic cancer (PC) cell evolution and carboplatin sensibility through controlling microRNA (miR)-495-3p.

[METHODS] LINC00963 and miR-495-3p levels in PC cells were tested via reverse transcription quantitative polymerase chain reaction (RT-qPCR). Cell counting kit (CCK)-8 assay monitored cell viability and half maximal inhibitory concentration (IC50) value for carboplatin. Cell clone number detection using colony formation assay and apoptosis rate detection using flow cytometry were proceeded. Dual luciferase reporter test verified the targeted relation of LINC00963 and miR-495-3p.

[RESULTS] LINC00963 enhancement and miR-495-3p weakening were found in PC cells. After interfering LINC00963, cell survival, clone numbers, and the IC50 value to carboplatin were decreased, and cell apoptosis rate and miR-495-3p abundance were increased. LINC00963 could reversely adjust miR-495-3p. Cell viability, clone numbers, and the IC50 value to carboplatin were declined, and apoptotic cell numbers were enhanced after miR-495-3p overexpression. The inhibition of miR-495-3p visibly mitigated the influence of LINC00963 interference on cell viability, clone numbers, apoptotic rate, and the IC50 value to carboplatin.

[CONCLUSION] LINC00963 downregulation could block PC cell progression and elevate their sensitivity to carboplatin through accelerating miR-495-3p level.