Dual targeting of BRAF and ferroptosis results in synergistic anticancer activity via iron overload and enhanced oxidative stress.
[BACKGROUND] While combination BRAF and MEK inhibitor treatment in -mutant cancers results in a response, treatment resistance and toxicity are common.
APA
Hu J, Ghosh C, et al. (2026). Dual targeting of BRAF and ferroptosis results in synergistic anticancer activity via iron overload and enhanced oxidative stress.. Journal of experimental & clinical cancer research : CR, 45(1), 34. https://doi.org/10.1186/s13046-025-03624-z
MLA
Hu J, et al.. "Dual targeting of BRAF and ferroptosis results in synergistic anticancer activity via iron overload and enhanced oxidative stress.." Journal of experimental & clinical cancer research : CR, vol. 45, no. 1, 2026, pp. 34.
PMID
41501927
Abstract
[BACKGROUND] While combination BRAF and MEK inhibitor treatment in -mutant cancers results in a response, treatment resistance and toxicity are common. Ferroptosis is an iron-dependent form of non-apoptotic cell death. BRAF inhibition has been associated with increased sensitivity to ferroptosis that is dependent on Glutathione Peroxidase 4 (GPX4).
[METHODS] In vitro, ex vivo, and in vivo models of anaplastic thyroid cancer (ATC) were used to evaluate the anticancer activity of combination BRAF inhibition and ferroptosis induction.
[RESULTS] Targeting key regulators of ferroptosis—GPX4, using RSL3 and ML162, and system X, using erastin—induced significant cell death in all ATC cell lines. Combination of dabrafenib and RSL3 synergistically increased cell death in -mutant ATC cells, and significantly inhibited colony formation. Mechanistically, lipid peroxidation, reactive oxygen species levels, and intracellular Fe increased significantly with combination treatment compared with each agent alone. Analysis of cell membrane iron importers and exporters showed significantly lower expression of ferroportin-1 (an iron exporter), suggesting the synergistic anticancer activity was due to increased iron accumulation and oxidative stress, leading to enhanced ferroptotic cell death. -mutant ATC cell spheroids showed synergistic cell death with dabrafenib and RSL3 treatment. In vivo, combination dabrafenib and ferroptosis induction (by targeting GPX4 using C18, and system X with IKE) significantly inhibited tumor growth in an orthotopic ATC mouse model. Additionally, dabrafenib-resistant -mutant ATC cells were more sensitive to ferroptosis induction than parental cells.
[CONCLUSIONS] Dual targeting of BRAFV600E and ferroptosis results in synergistic anticancer activity and overcomes resistance to BRAF inhibition.
[SUPPLEMENTARY INFORMATION] The online version contains supplementary material available at 10.1186/s13046-025-03624-z.
[METHODS] In vitro, ex vivo, and in vivo models of anaplastic thyroid cancer (ATC) were used to evaluate the anticancer activity of combination BRAF inhibition and ferroptosis induction.
[RESULTS] Targeting key regulators of ferroptosis—GPX4, using RSL3 and ML162, and system X, using erastin—induced significant cell death in all ATC cell lines. Combination of dabrafenib and RSL3 synergistically increased cell death in -mutant ATC cells, and significantly inhibited colony formation. Mechanistically, lipid peroxidation, reactive oxygen species levels, and intracellular Fe increased significantly with combination treatment compared with each agent alone. Analysis of cell membrane iron importers and exporters showed significantly lower expression of ferroportin-1 (an iron exporter), suggesting the synergistic anticancer activity was due to increased iron accumulation and oxidative stress, leading to enhanced ferroptotic cell death. -mutant ATC cell spheroids showed synergistic cell death with dabrafenib and RSL3 treatment. In vivo, combination dabrafenib and ferroptosis induction (by targeting GPX4 using C18, and system X with IKE) significantly inhibited tumor growth in an orthotopic ATC mouse model. Additionally, dabrafenib-resistant -mutant ATC cells were more sensitive to ferroptosis induction than parental cells.
[CONCLUSIONS] Dual targeting of BRAFV600E and ferroptosis results in synergistic anticancer activity and overcomes resistance to BRAF inhibition.
[SUPPLEMENTARY INFORMATION] The online version contains supplementary material available at 10.1186/s13046-025-03624-z.
같은 제1저자의 인용 많은 논문 (5)
- EZR-ROS1 rearrangement as a novel mechanism of acquired resistance to EGFR-TKIs in NSCLC: a case report and literature review.
- Epidemiological trends and characteristics of oral tongue cancer in females: systematic review and meta-analysis.
- Targeting ICMT: A promising strategy in cancer treatment (Review).
- Update on the clinical features, treatment and histogenesis of combined hepatocellular-cholangiocarcinoma.
- Integrating lymphovascular and perineural invasion into TNM staging: a novel ITNM system for enhanced prognostic stratification in colorectal cancer.