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An Inducible CRISPR-dCas9-Based Transcriptional Repression System for Cancer Therapy.

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Small methods 2024 Vol.8(8) p. e2301310
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Gu P, Zhao J, Zhang W, Ruan X, Hu L, Zeng Y

📝 환자 설명용 한 줄

Gene therapy has been adapted for improving malignant tumor treatment.

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↓ .bib ↓ .ris
APA Gu P, Zhao J, et al. (2024). An Inducible CRISPR-dCas9-Based Transcriptional Repression System for Cancer Therapy.. Small methods, 8(8), e2301310. https://doi.org/10.1002/smtd.202301310
MLA Gu P, et al.. "An Inducible CRISPR-dCas9-Based Transcriptional Repression System for Cancer Therapy.." Small methods, vol. 8, no. 8, 2024, pp. e2301310.
PMID 38164884 ↗

Abstract

Gene therapy has been adapted for improving malignant tumor treatment. However, pharmacotherapies targeting cancer remain limited and are generally inapplicable for rare disease patients. Oleanolic acid (OA) is a plant-derived triterpenoid that is frequently used in Chinese medicine as a safe but slow-acting treatment for many disorders. Here, the congruent pharmacological activities of OA and CRISPR-dCas9 in targeting AURKA or KDM1A and improving disease-specific prognosis and used a synthetic-biology-inspired design principle to engineer a therapeutic gene circuit that enables a concerted action of both drugs are utilized. In particular, the OA-triggered CRISPR-dCas9 transcriptional repression system rapidly and simultaneously attenuated lung and thyroid cancer. Collectively, this work shows that rationally engineered synthetic gene circuits are capable of treating multifactorial diseases in a synergistic manner by multiplexing the targeting efficiencies of single therapeutics.

🏷️ 키워드 / MeSH 📖 같은 키워드 OA만

🏷️ 같은 키워드 · 무료전문 — 이 논문 MeSH/keyword 기반