TRIB1 facilitates the proliferation and migration of ovarian cancer cells by inducing EMT progression.
[AIM] Ovarian cancer (OC) is a fatal female malignant tumor that severely impacts the health of women worldwide.
APA
Shi G, Holgersson K, et al. (2025). TRIB1 facilitates the proliferation and migration of ovarian cancer cells by inducing EMT progression.. Histology and histopathology, 40(9), 1447-1455. https://doi.org/10.14670/HH-18-868
MLA
Shi G, et al.. "TRIB1 facilitates the proliferation and migration of ovarian cancer cells by inducing EMT progression.." Histology and histopathology, vol. 40, no. 9, 2025, pp. 1447-1455.
PMID
39807763
Abstract
[AIM] Ovarian cancer (OC) is a fatal female malignant tumor that severely impacts the health of women worldwide. Due to the lack of diagnostic biomarkers, 70% of OC patients are considered in the advanced stage at the first diagnosis. Exploring novel biomarkers for OC diagnosis has become an urgent clinical need to address. TRIB1 is a newly discovered oncogene in several malignant tumors, including acute myeloid leukemia, prostate cancer, and breast cancer. However, the biological function of TRIB1 in OC remains uncertain and, therefore, was explored in the present study.
[METHODS] Levels of TRIB1 in OC and normal tissues were evaluated in the GEPIA database. TRIB1-KD was constructed in ES-2 cells and TRIB1-OE was constructed in OVCAR3 cells using a siRNA and OE vector, respectively. The proliferation ability was determined using the CCK-8 and clone formation assays. The migration ability was detected using the wound healing and Transwell assays. The expression of epithelial-mesenchymal transition (EMT) biomarkers was determined using western blotting.
[RESULTS] TRIB1 was markedly upregulated in OC tissues compared with normal ovarian tissues in the GEPIA database. The TRIB1 level was slightly altered among ES-2, CAOV3, and SKOV3 cells, with the highest expression in ES-2 cells, which was greatly reduced in OVCAR3 cells. In TRIB1-KD ES-2 cells, a remarkably reduced proliferation ability was observed with the CCK-8 and clone formation assays, accompanied by a reduction in migration distance in the Wound healing assay and the number of migrated cells in the Transwell assay. In contrast, in TRIB1-OE OVCAR3 cells, increased proliferation ability was observed, accompanied by increased migration distance and number of migrated cells. Furthermore, EMT progression was markedly repressed in TRIB1-KD ES-2 cells and remarkably enhanced in TRIB1-OE OVCAR3 cells.
[CONCLUSION] TRIB1 facilitated the proliferation and migration of OC cells by enhancing EMT progression.
[METHODS] Levels of TRIB1 in OC and normal tissues were evaluated in the GEPIA database. TRIB1-KD was constructed in ES-2 cells and TRIB1-OE was constructed in OVCAR3 cells using a siRNA and OE vector, respectively. The proliferation ability was determined using the CCK-8 and clone formation assays. The migration ability was detected using the wound healing and Transwell assays. The expression of epithelial-mesenchymal transition (EMT) biomarkers was determined using western blotting.
[RESULTS] TRIB1 was markedly upregulated in OC tissues compared with normal ovarian tissues in the GEPIA database. The TRIB1 level was slightly altered among ES-2, CAOV3, and SKOV3 cells, with the highest expression in ES-2 cells, which was greatly reduced in OVCAR3 cells. In TRIB1-KD ES-2 cells, a remarkably reduced proliferation ability was observed with the CCK-8 and clone formation assays, accompanied by a reduction in migration distance in the Wound healing assay and the number of migrated cells in the Transwell assay. In contrast, in TRIB1-OE OVCAR3 cells, increased proliferation ability was observed, accompanied by increased migration distance and number of migrated cells. Furthermore, EMT progression was markedly repressed in TRIB1-KD ES-2 cells and remarkably enhanced in TRIB1-OE OVCAR3 cells.
[CONCLUSION] TRIB1 facilitated the proliferation and migration of OC cells by enhancing EMT progression.
MeSH Terms
Humans; Female; Ovarian Neoplasms; Cell Proliferation; Cell Movement; Epithelial-Mesenchymal Transition; Protein Serine-Threonine Kinases; Cell Line, Tumor; Intracellular Signaling Peptides and Proteins; Gene Expression Regulation, Neoplastic; Biomarkers, Tumor; Disease Progression
같은 제1저자의 인용 많은 논문 (5)
- CD48 is a novel immune checkpoint on tumour-associated macrophages in hepatocellular carcinoma.
- B-Lymphoblastic Lymphoma Presenting With a Facial Mass as the Initial Symptom.
- GFRAL-Fc disarms GDF15 to reprogram tumor immunity and amplify PD-1 efficacy in hepatocellular carcinoma.
- Potential anti-gastric cancer properties of modified Lichong decoction based on metabolomics, network pharmacology, and pharmacological verification.
- Multiple thyroid disorders and risk of osteoporosis: a two-sample Mendelian randomization study.