Comparison of CRISPR Sequences in Archaea and Bacteria with Eukaryotic microRNAs.

[BACKGROUND] This study explores repetitive Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) sequences from the archaea and (), as well as from the bacterium (). These sequences are compared with human microRNA (miRNA) sequences to investigate potential genetic similarities and disease associations.

[METHODS] CRISPR sequences were retrieved from the CRISPR/Cas database, and human miRNA sequences were obtained from miRBase. Sequence alignments were performed using BLASTn with an E-value threshold of 1e-5 to identify significant similarities. Genes associated with matched human miRNAs were identified through the HGNC and GeneCards databases. Further analyses included comparison with disease-associated miRNAs reported in human and mouse datasets.

[RESULTS] In , alignments revealed similarities to miRNAs linked with genes such as , , , and , which are associated with lung cancer and muscular dystrophies. In , aligned miRNAs corresponded to loci including and potentially linked to periembolic adenocarcinoma and mild pre-eclampsia. For , matches were observed with miRNAs associated with genes like , , , and , which have been implicated in Psoriatic arthritis, Alzheimer's disease, Hepatocellular carcinoma, and Coronary artery disease.

[CONCLUSION] CRISPR sequences from these prokaryotes show notable similarities with human miRNAs, suggesting possible indirect links to genes involved in major diseases. These preliminary findings emphasize the need for further investigation into shared sequence motifs and their functional roles in host-pathogen interactions or evolutionary biology.