Fabrication and analysis of polydatin-loaded gelatin-coated Polycaprolactone nanoparticles for targeting radioresistant lung cancer cells.

Polydatin a well know phytochemical, with biological activities ranging from antioxidant, anti-inflammatory, to anticancer, modulates various signal transduction pathways in lung cancer. Despite several advantages the therapeutic potential of polydatin is limited due to its poor absorption and low bioavailability. To address these challenges nanoparticle-based therapy has emerged as a revolutionary method in the management of many disease including lung cancer. In the present study we have designed, synthesized and characterized the bipolymeric polydatin nanoformuations. The bioefficacy of nanoparticles was studied on A549 and A549 radioresistant lung cancer cell lines in-vitro both on 2D and 3D spheroids cultures and validated using proteomic and in silico analysis. The molecular targets for radioresistant lung cancer cells, their interaction with polydatin and signalling pathways involved were deciphered.The mechanism of action of PPG-NPs was almost similar to polydatin, however, few differences resulted in increased efficacy.