Biochanin A, an isoflavone isolated from Roxb. ex DC., leaves promote ROS-mediated and caspase-dependent apoptosis in lung adenocarcinoma cells.
기술보고
1/5 보강
PICO 자동 추출 (휴리스틱, conf 2/4)
유사 논문P · Population 대상 환자/모집단
Through comprehensive characterization, a cytotoxic compound called Biochanin A (BA) was identified, exhibiting significant anticancer activity with an IC value of 21.92 ± 2.19 μM against A549 cells, while demonstrating lower cytotoxicity …
I · Intervention 중재 / 시술
추출되지 않음
C · Comparison 대조 / 비교
추출되지 않음
O · Outcome 결과 / 결론
As a result, BA's molecular mechanism of action involves ROS-induced apoptosis mediated by caspases 9 and 3.
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The objective of this study was to isolate and characterize a cytotoxic compound from the hydromethanolic extract of Roxb.
APA
Thakkar AB, Subramanian RB, et al. (2026). Biochanin A, an isoflavone isolated from Roxb. ex DC., leaves promote ROS-mediated and caspase-dependent apoptosis in lung adenocarcinoma cells.. Journal of biomolecular structure & dynamics, 44(5), 2328-2352. https://doi.org/10.1080/07391102.2025.2507820
MLA
Thakkar AB, et al.. "Biochanin A, an isoflavone isolated from Roxb. ex DC., leaves promote ROS-mediated and caspase-dependent apoptosis in lung adenocarcinoma cells.." Journal of biomolecular structure & dynamics, vol. 44, no. 5, 2026, pp. 2328-2352.
PMID
40432355 ↗
Abstract 한글 요약
The objective of this study was to isolate and characterize a cytotoxic compound from the hydromethanolic extract of Roxb. ex DC. leaves using the cold percolation technique. Thin-layer chromatography was employed to isolate the cytotoxic component from the crude plant extract, and its cytotoxicity against lung adenocarcinoma (A549) cells was evaluated using the MTT assay. The structure of the isolated cytotoxic compound was determined through FTIR, NMR, UV analysis, and LC-MS/MS methods. Through comprehensive characterization, a cytotoxic compound called Biochanin A (BA) was identified, exhibiting significant anticancer activity with an IC value of 21.92 ± 2.19 μM against A549 cells, while demonstrating lower cytotoxicity towards normal lung cells (WI-38) with an IC value of 285.12 ± 2.19 μM. Notably, BA induced morphological changes in A549 cells, leading to apoptotic alterations and the generation of reactive oxygen species (ROS), as confirmed by multiple techniques (AO/EB, DAPI, Giemsa). molecular docking, ADMET, MMGBSA, and molecular dynamics simulation investigations support the RT-PCR and cell biology findings. As a result, BA's molecular mechanism of action involves ROS-induced apoptosis mediated by caspases 9 and 3.
🏷️ 키워드 / MeSH 📖 같은 키워드 OA만
- Humans
- Apoptosis
- Reactive Oxygen Species
- Plant Leaves
- Genistein
- Molecular Docking Simulation
- Dalbergia
- Adenocarcinoma of Lung
- A549 Cells
- Plant Extracts
- Caspases
- Lung Neoplasms
- Cell Line
- Tumor
- Isoflavones
- Antineoplastic Agents
- Phytogenic
- A549
- Biochanin A
- Cytotoxicity
- Dalbergia sissoo Roxb. ex DC
- apoptosis
🏷️ 같은 키워드 · 무료전문 — 이 논문 MeSH/keyword 기반
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