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p53 mediated cell cytotoxicity and DNA damage in lung cancer cell line A549 triggered by a small peptide extracted from a probiotic strain Lacticaseibacillus casei.

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BMC biotechnology 2026 Vol.26(1)
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Siddique JF, Kumari GRS, Kumar SDV, Devi CS, Vaithilingam M

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[BACKGROUND] Antimicrobial peptides are being explored as anticancer agents due to their selectivity toward transformed cells.

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APA Siddique JF, Kumari GRS, et al. (2026). p53 mediated cell cytotoxicity and DNA damage in lung cancer cell line A549 triggered by a small peptide extracted from a probiotic strain Lacticaseibacillus casei.. BMC biotechnology, 26(1). https://doi.org/10.1186/s12896-026-01119-z
MLA Siddique JF, et al.. "p53 mediated cell cytotoxicity and DNA damage in lung cancer cell line A549 triggered by a small peptide extracted from a probiotic strain Lacticaseibacillus casei.." BMC biotechnology, vol. 26, no. 1, 2026.
PMID 41826945 ↗

Abstract

[BACKGROUND] Antimicrobial peptides are being explored as anticancer agents due to their selectivity toward transformed cells. We evaluated a small peptide from for anti-proliferative effects on A549 lung cancer cells and investigated apoptosis-related mechanisms in vitro.

[METHODS] Cell viability was assessed by MTT. Apoptosis-associated morphology was examined by AO/EB staining, DNA fragmentation by agarose gel electrophoresis, and gene expression by qRT-PCR (p53, BAX, BCL-2, CASP8, CASP7, CASP3).

[RESULTS] The peptide reduced A549 viability in a dose-dependent manner relative to L929 cells. AO/EB staining revealed early apoptotic nuclear morphology, and DNA fragmentation was observed. qRT-PCR showed , , , and .

[CONCLUSIONS] Data support apoptosis that is with . As per experimental evidence of the study, the peptide from has the potential to inhibit the proliferation of Lung cancer cell line A549.

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