Ionizing radiation enhances prognostically significant cellular immunity programs in the brain metastasis microenvironment.
[PURPOSE] Brain metastasis (BM) is a deadly complication of systemic malignancy, which has been associated with defective cellular immunity.
- 표본수 (n) 153
APA
Fukumura K, Jiang P, et al. (2026). Ionizing radiation enhances prognostically significant cellular immunity programs in the brain metastasis microenvironment.. Clinical cancer research : an official journal of the American Association for Cancer Research. https://doi.org/10.1158/1078-0432.CCR-25-3525
MLA
Fukumura K, et al.. "Ionizing radiation enhances prognostically significant cellular immunity programs in the brain metastasis microenvironment.." Clinical cancer research : an official journal of the American Association for Cancer Research, 2026.
PMID
41817317
Abstract
[PURPOSE] Brain metastasis (BM) is a deadly complication of systemic malignancy, which has been associated with defective cellular immunity. We sought to characterize the foundational elements and clinical relevance BM-associated immunosuppression using integrated molecular profiling in primary patient material.
[EXPERIMENTAL DESIGN] Retrospective patient tissue cohorts of breast (N=153) and lung (N=153) cancer BM were stratified by histopathological scoring of tumor-infiltrating lymphocytes (TILs) and clinical outcome, with a large subset of breast cancer samples further analyzed by T-cell receptor (TCR) and RNA sequencing. An ongoing clinical trial comparing pre-operative (pre-op) and post-operative (post-op) stereotactic radiosurgery (SRS)/stereotactic radiotherapy (SRT) in BM management was then leveraged to dissect radiation-induced immune responses by TCR and RNA sequencing.
[RESULTS] Patients with high-grade histopathological TIL infiltration and enriched TCR diversity demonstrated favorable prognoses in breast and lung cancer BM. Moreover, SRS/SRT treatment enhanced TCR diversity in the BM microenvironment, along with signatures of antigen processing and presentation. Finally, integrated analysis demonstrated that IR appeared to reactivate immune microenvironmental signatures normally suppressed in BM and upregulate immune signaling pathways correlated with favorable outcome in breast cancer BM patients.
[CONCLUSIONS] Our findings demonstrate that high TCR diversity in BM associates with favorable prognosis, pointing to therapeutically tractable targets within the immune microenvironment. Moreover, we show in a prospective clinical trial that IR enhances T cell-mediated immune responses, upregulating antigen-presentation and enhancing TCR diversity in BM. These results argue for increased therapeutic investigations of radiation-induced immunomodulatory effects in BM, potentially in association with immune checkpoint inhibition.
[EXPERIMENTAL DESIGN] Retrospective patient tissue cohorts of breast (N=153) and lung (N=153) cancer BM were stratified by histopathological scoring of tumor-infiltrating lymphocytes (TILs) and clinical outcome, with a large subset of breast cancer samples further analyzed by T-cell receptor (TCR) and RNA sequencing. An ongoing clinical trial comparing pre-operative (pre-op) and post-operative (post-op) stereotactic radiosurgery (SRS)/stereotactic radiotherapy (SRT) in BM management was then leveraged to dissect radiation-induced immune responses by TCR and RNA sequencing.
[RESULTS] Patients with high-grade histopathological TIL infiltration and enriched TCR diversity demonstrated favorable prognoses in breast and lung cancer BM. Moreover, SRS/SRT treatment enhanced TCR diversity in the BM microenvironment, along with signatures of antigen processing and presentation. Finally, integrated analysis demonstrated that IR appeared to reactivate immune microenvironmental signatures normally suppressed in BM and upregulate immune signaling pathways correlated with favorable outcome in breast cancer BM patients.
[CONCLUSIONS] Our findings demonstrate that high TCR diversity in BM associates with favorable prognosis, pointing to therapeutically tractable targets within the immune microenvironment. Moreover, we show in a prospective clinical trial that IR enhances T cell-mediated immune responses, upregulating antigen-presentation and enhancing TCR diversity in BM. These results argue for increased therapeutic investigations of radiation-induced immunomodulatory effects in BM, potentially in association with immune checkpoint inhibition.