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Molecular analysis of tumor recurrence using established cancer stem cell-line and drug discovery.

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International journal of clinical oncology 📖 저널 OA 27.8% 2021: 0/1 OA 2022: 0/3 OA 2023: 1/2 OA 2024: 2/9 OA 2025: 11/57 OA 2026: 26/68 OA 2021~2026 2026 Vol.31(3) p. 393-403
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Nakano K, Oki E, Yamazaki M, Suzuki M, Kawai S, Zaitsu Y

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Despite advances in adjuvant therapy for colorectal cancer, tumor relapses, often driven by minimal residual disease, remain a formidable clinical challenge.

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APA Nakano K, Oki E, et al. (2026). Molecular analysis of tumor recurrence using established cancer stem cell-line and drug discovery.. International journal of clinical oncology, 31(3), 393-403. https://doi.org/10.1007/s10147-025-02948-2
MLA Nakano K, et al.. "Molecular analysis of tumor recurrence using established cancer stem cell-line and drug discovery.." International journal of clinical oncology, vol. 31, no. 3, 2026, pp. 393-403.
PMID 41557221 ↗

Abstract

Despite advances in adjuvant therapy for colorectal cancer, tumor relapses, often driven by minimal residual disease, remain a formidable clinical challenge. The cancer stem cell hypothesis provides a key framework for understanding this problem, positing that a small, therapy-resistant subpopulation of cells drives recurrence. To elucidate the role of these cells, we developed LGR5-specific monoclonal antibodies and a high-sensitivity immunofluorescence method to visualize the stem cell marker LGR5 in clinical tumors. Furthermore, we established a unique colorectal cancer cell line, PLR123, which maintains robust stem cell properties, and developed an in vitro model to study tumor recurrence. Through analyses including single-cell RNA sequencing and small molecule screening, we identified the RNA Polymerase I inhibitor BMH-21 as a compound that effectively suppresses recurrence both in vitro and in vivo. This article comprehensively reviews our series of studies on understanding the mechanisms of cancer stem cell-driven resistance and offers insights supporting the development of novel therapies aimed at preventing tumor relapses.

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