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Poly-thymine-mediated fluorescence recovery of N-CQDs for turn-on sensing of APE1 activity in breast cancer cell lysates.

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Analytical methods : advancing methods and applications 2026 Advanced biosensing and bioanalysis
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PubMed DOI OpenAlex 마지막 보강 2026-04-29
OpenAlex 토픽 · Advanced biosensing and bioanalysis techniques Epigenetics and DNA Methylation DNA Repair Mechanisms

Xiong X, Li Y, Zhao M, Zhao J, Xiong S, Sun Z

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A label-free turn-on fluorescent biosensor is developed for APE1 activity detection based on APE1-gated release of poly-T strands from a constrained DNA duplex, which competitively displaces Ag from N

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BibTeX ↓ RIS ↓
APA Xinjuan Xiong, Yanning Li, et al. (2026). Poly-thymine-mediated fluorescence recovery of N-CQDs for turn-on sensing of APE1 activity in breast cancer cell lysates.. Analytical methods : advancing methods and applications. https://doi.org/10.1039/d6ay00468g
MLA Xinjuan Xiong, et al.. "Poly-thymine-mediated fluorescence recovery of N-CQDs for turn-on sensing of APE1 activity in breast cancer cell lysates.." Analytical methods : advancing methods and applications, 2026.
PMID 42028838
DOI 10.1039/d6ay00468g

Abstract

A label-free turn-on fluorescent biosensor is developed for APE1 activity detection based on APE1-gated release of poly-T strands from a constrained DNA duplex, which competitively displaces Ag from N-CQDs, recovering fluorescence. The sensor achieves a detection limit of 0.02 U mL, excellent selectivity, and one-step operation. It successfully detects APE1 in serum (recoveries 92-108%) and in cancer cell lysates (MCF-7, MDA-MB-231, HeLa, HepG2), with a threshold as low as 192 cells, and distinguishes cancer cells from normal cells. This simple, robust platform holds promise for DNA repair research, cancer diagnostics, and inhibitor screening.

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