Nanobody-Functionalized Magnetic Beads for Enrichment of Foodborne Botulinum Toxin Type A Light Chain.
Abstract
In this study, for the first time, nanobody conjugated beads were developed to enrich botulinum serotype A. We evaluated two types of nanobody A8-functionalized magnetic beads, both of which effectively captured and enriched BoNT/A from dilute solutions and complex protein mixtures, achieving a concentration increase of approximately 12- to 30-fold. The A8@N-beads showed higher binding capacity than the A8@S-beads, suggesting that our self-synthesized NH beads work well in this application. Our data serve as evidence that using different bead types with nanobody conjugation is a promising enrichment approach for further detection of Lc/A, particularly in applications involving low-abundance targets or complex sample matrices, such as food safety screening or clinical diagnostics.
추출된 의학 개체 (NER)
| 유형 | 영어 표현 | 한국어 / 풀이 | UMLS CUI | 출처 | 등장 |
|---|---|---|---|---|---|
| 시술 | botulinum toxin
|
보툴리눔독소 주사 | dict | 1 | |
| 기타 | Foodborne Botulinum Toxin Type A Light Chain
|
scispacy | 1 | ||
| 기타 | nanobody conjugated beads
|
scispacy | 1 | ||
| 기타 | botulinum serotype A.
|
scispacy | 1 | ||
| 기타 | BoNT/A
|
scispacy | 1 |
MeSH Terms
Botulinum Toxins, Type A; Single-Domain Antibodies; Food Microbiology; Microspheres; Clostridium botulinum; Immunomagnetic Separation
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