An efficient method for eccrine gland isolation from human scalp.

Experimental dermatology 2018 Vol.27(6) p. 678-681

Jimenez F, Alam M, Hernandez I, Poblet E, Hardman JA, Paus R

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Abstract

We describe a simple and efficient method to isolate eccrine sweat glands from the human scalp. This method is inspired by the hair graft harvesting method used in hair transplantation. Based on the recently described anatomical relationship between the scalp hair follicle and the eccrine gland, we have found that scalp follicular unit grafts are an excellent eccrine gland isolation source, especially for the coiled component. In order to make the gland visible for stereoscopic microdissection, the follicular units need to be previously stained with a vital dye like methylene blue or neutral red. The simplicity and efficiency of this isolation method should encourage further research into human eccrine sweat gland function which has always been hindered by the difficulty of gland isolation.

추출된 의학 개체 (NER)

유형영어 표현한국어 / 풀이UMLS CUI출처등장
시술 hair transplantation 모발이식 dict 1
해부 hair scispacy 1
해부 grafts scispacy 1
해부 gland scispacy 1
합병증 eccrine gland scispacy 1
합병증 scalp follicular scispacy 1
약물 methylene blue C0025746
methylene blue
scispacy 1
기타 human scalp scispacy 1
기타 eccrine sweat glands scispacy 1
기타 hair graft scispacy 1
기타 scalp hair follicle scispacy 1
기타 follicular scispacy 1
기타 human eccrine sweat gland scispacy 1

MeSH Terms

Coloring Agents; Eccrine Glands; Hair Follicle; Humans; Methylene Blue; Microdissection; Neutral Red; Scalp; Staining and Labeling; Tissue and Organ Harvesting

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