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In vitro study of the effects of ELF electric fields on gene expression in human epidermal cells.

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Bioelectromagnetics 2011 Vol.32(1) p. 28-36
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Collard JF, Mertens B, Hinsenkamp M

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📝 환자 설명용 한 줄

An acceleration of differentiation, at the expense of proliferation, is observed after exposure of various biological models to low frequency and low amplitude electric and electromagnetic fields.

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APA Collard JF, Mertens B, Hinsenkamp M (2011). In vitro study of the effects of ELF electric fields on gene expression in human epidermal cells.. Bioelectromagnetics, 32(1), 28-36. https://doi.org/10.1002/bem.20608
MLA Collard JF, et al.. "In vitro study of the effects of ELF electric fields on gene expression in human epidermal cells.." Bioelectromagnetics, vol. 32, no. 1, 2011, pp. 28-36.
PMID 20809503 ↗
DOI 10.1002/bem.20608

Abstract

An acceleration of differentiation, at the expense of proliferation, is observed after exposure of various biological models to low frequency and low amplitude electric and electromagnetic fields. Following these results showing significant modifications, we try to identify the biological mechanism involved at the cell level through microarray screening. For this study, we use epidermis cultures harvested from human abdominoplasty. Two platinum electrodes are used to apply the electric signal. The gene expressions of 38,500 well-characterized human genes are analyzed using Affymetrix(®) microarray U133 Plus 2.0 chips. The protocol is repeated on three different patients. After three periods of exposure, a total of 24 chips have been processed. After the application of ELF electric fields, the microarray analysis confirms a modification of the gene expression of epidermis cells. Particularly, four up-regulated genes (DKK1, TXNRD1, ATF3, and MME) and one down-regulated gene (MACF1) are involved in the regulation of proliferation and differentiation. Expression of these five genes was also confirmed by real-time rtPCR in all samples used for microarray analysis. These results corroborate an acceleration of cell differentiation at the expense of cell proliferation.

추출된 의학 개체 (NER)

전체 NER 표 보기
유형영어 표현한국어 / 풀이UMLS CUI출처등장
시술 abdominoplasty 복부성형술 dict 1
해부 cell scispacy 1
해부 epidermis cultures scispacy 1
해부 epidermis cells scispacy 1
약물 platinum C0032207
platinum
scispacy 1
기타 human epidermal cells scispacy 1
기타 human abdominoplasty scispacy 1
기타 human scispacy 1
기타 DKK1 scispacy 1
기타 TXNRD1 scispacy 1
기타 ATF3 scispacy 1
기타 MME scispacy 1
기타 MACF1 scispacy 1

🏷️ 키워드 / MeSH 📖 같은 키워드 OA만

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🏷️ 같은 키워드 · 무료전문 — 이 논문 MeSH/keyword 기반

📖 비슷한 OA 논문 — 같은 카테고리, 무료 전문 가능