Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation.
Adipose-derived stromal cells (ASC) are a promising alternative cell source to chondrocytes as well as to bone marrow-derived mesenchymal stromal cells (BMSC) in cartilage tissue engineering and repai
APA
Buchert JM, Lotz B, et al. (2023). Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation.. Methods in molecular biology (Clifton, N.J.), 2598, 75-85. https://doi.org/10.1007/978-1-0716-2839-3_7
MLA
Buchert JM, et al.. "Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation.." Methods in molecular biology (Clifton, N.J.), vol. 2598, 2023, pp. 75-85.
PMID
36355286
Abstract
Adipose-derived stromal cells (ASC) are a promising alternative cell source to chondrocytes as well as to bone marrow-derived mesenchymal stromal cells (BMSC) in cartilage tissue engineering and repair. Here we describe ASC isolation from liposuction by-products by collagenase-based tissue digestion combined with cell filtration and followed by monolayer attachment and expansion culture. Quality control requires confirmation of correct surface marker expression and multilineage differentiation potential by a trilineage differentiation assay.
추출된 의학 개체 (NER)
| 유형 | 영어 표현 | 한국어 / 풀이 | UMLS CUI | 출처 | 등장 |
|---|---|---|---|---|---|
| 시술 | liposuction
|
지방흡입 | dict | 1 | |
| 해부 | Adipose-Derived Stromal Cells
|
scispacy | 1 | ||
| 해부 | ASC
→ Adipose-derived stromal cells
|
scispacy | 1 | ||
| 해부 | cell
|
scispacy | 1 | ||
| 해부 | chondrocytes
|
scispacy | 1 | ||
| 해부 | bone marrow-derived mesenchymal stromal cells
|
scispacy | 1 | ||
| 해부 | BMSC
→ bone marrow-derived mesenchymal stromal cells
|
scispacy | 1 | ||
| 해부 | cartilage tissue
|
scispacy | 1 | ||
| 해부 | tissue
|
scispacy | 1 | ||
| 해부 | monolayer
|
scispacy | 1 | ||
| 해부 | surface
|
scispacy | 1 | ||
| 약물 | BMSC
→ bone marrow-derived mesenchymal stromal cells
|
scispacy | 1 |
MeSH Terms
Chondrogenesis; Adipose Tissue; Cell Differentiation; Stromal Cells; Cartilage; Chondrocytes; Cells, Cultured; Bone Marrow Cells
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