Growth of normal human mammary cells in culture.
Abstract
Reduction mammoplasty tissue was used to obtain short-term cultures of human epithelial cell populations. Digestion of tissue with collagenase and hyaluronidase resulted in cell clusters (organoids) resembling ductal and alveolar structures; these could be separated by filtration from the stromal components. Epithelial outgrowth from these organoids was greatly enhanced by the addition of conditioned medium from other human epithelial and myoepithelial cell lines. Additionally, the mammary epithelial growth was stimulated by insulin, hydrocortisone, epidermal growth factor, and steroid hormones. With this enriched nutritional environment, active cell division could be maintained for 1 to 3 months and cells could be serially subcultured 1 to 4 times.
추출된 의학 개체 (NER)
| 유형 | 영어 표현 | 한국어 / 풀이 | UMLS CUI | 출처 | 등장 |
|---|---|---|---|---|---|
| 해부 | mammary
|
유방 | dict | 2 | |
| 시술 | reduction mammoplasty
|
유방성형술 | dict | 1 | |
| 해부 | tissue
|
scispacy | 1 | ||
| 해부 | cell
|
scispacy | 1 | ||
| 해부 | organoids
|
scispacy | 1 | ||
| 해부 | stromal
|
scispacy | 1 | ||
| 해부 | myoepithelial cell lines
|
scispacy | 1 | ||
| 해부 | mammary epithelial
|
scispacy | 1 | ||
| 해부 | cells
|
scispacy | 1 | ||
| 약물 | hydrocortisone
|
C0020268
hydrocortisone
|
scispacy | 1 | |
| 약물 | steroid
|
C0038317
Steroids
|
scispacy | 1 | |
| 기타 | human mammary cells
|
scispacy | 1 | ||
| 기타 | human epithelial cell populations
|
scispacy | 1 | ||
| 기타 | collagenase
|
scispacy | 1 | ||
| 기타 | hyaluronidase
|
scispacy | 1 | ||
| 기타 | alveolar
|
scispacy | 1 | ||
| 기타 | human epithelial
|
scispacy | 1 | ||
| 기타 | insulin
|
scispacy | 1 | ||
| 기타 | epidermal growth factor
|
scispacy | 1 |
MeSH Terms
Breast; Cell Division; Cells, Cultured; Culture Media; Epidermal Growth Factor; Epithelial Cells; Female; Fibroblasts; Growth Substances; Hormones; Humans; Hydrocortisone; Insulin
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