Reconstitution of hair follicle development in vivo: determination of follicle formation, hair growth, and hair quality by dermal cells.
💡 한 문장 핵심
생체 내 모낭 발달의 재구성: 진피세포에 의한 모낭 형성, 모발 성장 및 모발 품질의 결정.
5/5 보강
TL;DR
This reconstitution model should be useful for identifying cell populations within the hair follicles compartment necessary for hair growth and for examining the effects of specific gene products on hair follicle growth and development in vivo.
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연도별 인용 (2012–2025) · 합계 109
OpenAlex 토픽 ·
Hair Growth and Disorders
Skin and Cellular Biology Research
Dermatologic Treatments and Research
🇰🇷 한글 요약 🌐 Abstract
【연구 목적】
무모 누드마우스(athymic nude mouse) 등에 신생/주산기 마우스의 표피·진피 세포 조합을 이식하여, 모낭(hair follicle) 형성·모발 성장·모발 질(quality)을 좌우하는 세포학적 요건을 규명하고자 함.
【방법】
트립신 분리 표피 각질세포(keratinocyte), 표피에서 분리한 발생 중 모낭아(hair follicle bud), 진피에서 분리한 전형성 모낭(pre-formed follicle) 등 다양한 표피 분획을 신선(fresh) 또는 배양(cultured) 진피세포와 조합하여 누드마우스 등에 이식. 추가로 쥐 수염(vibrissa) 모낭에서 미세박리한 모유두세포(dermal papilla cell)를 최대 14계대까지 배양해 이식 실험을 수행함.
【주요 결과】
모든 표피 분획은 신선 진피세포와 함께 이식 시 유모 피부(haired skin)를 형성했으나, 전형성 모낭만이 진피세포 없이도 단독으로 모발을 생성함. 모낭아는 단독 또는 배양 진피세포와 이식 시 피부는 재형성되나 모낭은 형성되지 않아, 신선 진피세포에만 존재하는 인자가 모낭아 단계의 모발 성장에 필수임. 배양 모유두세포는 14계대까지도 유도능(inductive capacity)을 유지하여 모낭아의 모발 성장을 자극하고, 전형성 모낭의 모발 질에도 영향을 미침.
【임상적 시사점 (성형외과 의사 관점)】
모유두세포는 장기 배양 후에도 유도능을 보존하므로, 자가 모유두세포 배양·이식을 활용한 모낭 재생(hair follicle neogenesis) 기반 탈모 치료의 이론적 근거가 됨. 다만 미성숙 모낭아 단계에서는 "신선 진피세포 유래 인자"가 필수적이어서, 임상 적용 시 배양 조건 최적화와 진피 미세환경(niche) 재현이 모발이식(hair transplantation)·세포치료제 개발의 핵심 과제임을 시사함.
Combinations of cultured and uncultured epidermal and dermal cell preparations from newborn and perinatal mice were grafted onto the backs of athymic nude mouse hosts to elucidate the cellular requirements for skin appendage formation. All epidermal populations studied, including a total epidermal keratinocyte preparation from trypsin-split skin, developing hair follicle buds isolated from epidermis, and preformed hair follicles isolated from dermis, make haired skin when grafted with fresh dermal cells. Only pre-formed hair follicles produce haired skin on grafts without an additional dermal component. Hair follicle buds grafted alone or with cultured dermal cells will reconstitute skin but without appendage formation. Thus, cells or factors present in fresh, but not cultured, dermal cells are essential for supporting hair growth from budding follicles, whereas more developed (pre-formed) follicles appear to contain all the necessary components for hair formation. Dissociation of isolated hair follicles by trypsin/ethylenediaminetetraacetic acid prior to grafting is permissive for hair growth, suggesting that follicle cells can be re-induced or reassociate in vivo. Dermal papilla cells, microdissected from rat vibrissal follicles and cultured for up to 14 passages, stimulate hair growth from follicle buds and influence the quality of hair growth from pre-formed hair follicles. Thus, dermal papilla cells maintain inductive capacity in culture and contribute to the reconstituted skin. This reconstitution model should be useful for identifying cell populations within the hair follicle compartment necessary for hair growth and for examining the effects of specific gene products on hair follicle growth and development in vivo.
【연구 목적】 무모 누드마우스(athymic nude mouse) 등에 신생/주산기 마우스의 표피·진피 세포 조합을 이식하여, 모낭(hair follicle) 형성·모발 성장·모발 질(quality)을 좌우하는 세포학적 요건을 규명하고자 함.
APA 7
Weinberg, W. C., Goodman, L. V., George, C., Morgan, D. L., Ledbetter, S., Yuspa, S. H., & Lichti, U. (1993). Reconstitution of hair follicle development in vivo: Determination of follicle formation, hair growth, and hair quality by dermal cells.. The Journal of investigative dermatology, 100(3), 229-36. https://doi.org/10.1111/1523-1747.ep12468971
Vancouver
Weinberg WC, Goodman LV, George C, Morgan DL, Ledbetter S, Yuspa SH, et al. Reconstitution of hair follicle development in vivo: determination of follicle formation, hair growth, and hair quality by dermal cells. Jour. inve. derm.. 1993;100(3):229-36. doi:10.1111/1523-1747.ep12468971
AMA 11
Weinberg WC, Goodman LV, George C, Morgan DL, Ledbetter S, Yuspa SH, et al. Reconstitution of hair follicle development in vivo: determination of follicle formation, hair growth, and hair quality by dermal cells. Jour. inve. derm.. 1993;100(3):229-36. doi:10.1111/1523-1747.ep12468971
Chicago
Weinberg, W. C., Goodman, L. V., George, C., Morgan, D. L., Ledbetter, S., Yuspa, S. H., and Lichti, U.. 1993. "Reconstitution of hair follicle development in vivo: determination of follicle formation, hair growth, and hair quality by dermal cells." The Journal of investigative dermatology 100 (3): 229-36. https://doi.org/10.1111/1523-1747.ep12468971
MLA 9
Weinberg, W. C., et al. "Reconstitution of hair follicle development in vivo: determination of follicle formation, hair growth, and hair quality by dermal cells." The Journal of investigative dermatology, vol. 100, no. 3, 1993, pp. 229-36. doi:10.1111/1523-1747.ep12468971.
PMID
8440892 ↗
🏷️ 키워드 / MeSH 📖 같은 키워드 OA만
인용 관계
그래프 OA 노드: 8/8 (100%)
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