A single-cell atlas of the healthy breast tissues reveals clinically relevant clusters of breast epithelial cells.
Abstract
Single-cell RNA sequencing (scRNA-seq) is an evolving technology used to elucidate the cellular architecture of adult organs. Previous scRNA-seq on breast tissue utilized reduction mammoplasty samples, which are often histologically abnormal. We report a rapid tissue collection/processing protocol to perform scRNA-seq of breast biopsies of healthy women and identify 23 breast epithelial cell clusters. Putative cell-of-origin signatures derived from these clusters are applied to analyze transcriptomes of ~3,000 breast cancers. Gene signatures derived from mature luminal cell clusters are enriched in ~68% of breast cancers, whereas a signature from a luminal progenitor cluster is enriched in ~20% of breast cancers. Overexpression of luminal progenitor cluster-derived signatures in HER2+, but not in other subtypes, is associated with unfavorable outcome. We identify TBX3 and PDK4 as genes co-expressed with estrogen receptor (ER) in the normal breasts, and their expression analyses in >550 breast cancers enable prognostically relevant subclassification of ER+ breast cancers.
추출된 의학 개체 (NER)
| 유형 | 영어 표현 | 한국어 / 풀이 | UMLS CUI | 출처 | 등장 |
|---|---|---|---|---|---|
| 해부 | breast
|
유방 | dict | 10 | |
| 시술 | reduction mammoplasty
|
유방성형술 | dict | 1 | |
| 해부 | breast tissues
|
scispacy | 1 | ||
| 해부 | breast epithelial cells
|
scispacy | 1 | ||
| 해부 | Single-cell
|
scispacy | 1 | ||
| 해부 | cellular
|
scispacy | 1 | ||
| 해부 | organs
|
scispacy | 1 | ||
| 해부 | scRNA-seq
→ Single-cell RNA sequencing
|
scispacy | 1 | ||
| 해부 | tissue
|
scispacy | 1 | ||
| 해부 | breast epithelial cell
|
scispacy | 1 | ||
| 합병증 | luminal progenitor
|
scispacy | 1 | ||
| 약물 | luminal
|
C0524462
Luminal region
|
scispacy | 1 | |
| 약물 | estrogen
|
C0014939
estrogens
|
scispacy | 1 | |
| 질환 | ER+ breast cancers
|
C0006142
Malignant neoplasm of breast
|
scispacy | 1 | |
| 질환 | scRNA-seq
→ Single-cell RNA sequencing
|
scispacy | 1 | ||
| 질환 | breast tissue
|
scispacy | 1 | ||
| 질환 | breast biopsies
|
scispacy | 1 | ||
| 질환 | cell-of-origin
|
scispacy | 1 | ||
| 질환 | breasts
|
scispacy | 1 | ||
| 질환 | breast cancers
|
C0006142
Malignant neoplasm of breast
|
scispacy | 1 | |
| 기타 | women
|
scispacy | 1 | ||
| 기타 | HER2
|
scispacy | 1 | ||
| 기타 | TBX3
|
scispacy | 1 | ||
| 기타 | PDK4
|
scispacy | 1 | ||
| 기타 | estrogen receptor
|
scispacy | 1 |
MeSH Terms
Adult; Atlases as Topic; Breast Neoplasms; Cell Lineage; Epithelial Cells; Estrogen Receptor alpha; Female; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; High-Throughput Nucleotide Sequencing; Humans; Mammary Glands, Human; Prognosis; Pyruvate Dehydrogenase Acetyl-Transferring Kinase; Erb-b2 Receptor Tyrosine Kinases; Signal Transduction; Single-Cell Analysis; Stem Cells; Survival Analysis; T-Box Domain Proteins; Transcriptome
🔗 함께 등장하는 도메인
이 논문이 속한 카테고리와 같은 논문에서 자주 함께 다뤄지는 카테고리들
관련 논문
- The impact of three-dimensional simulation and virtual reality technologies on surgical decision-making and postoperative satisfaction in aesthetic surgery: a preliminary study.
- Cutaneous fistula of the breast: A complication of cosmetic autologous fat transfer.
- Epidermal inclusion cyst after breast reduction mammoplasty.
- Clinical outcomes of synthetic absorbable mesh use in breast surgery: First case series in reconstruction and aesthetic mastopexy.
- Implant-based versus autologous mastopexy after massive weight loss: Complications and patient satisfaction.