Bone Marrow Basophil Evaluation in Myeloproliferative Neoplasms Using Flow Cytometry.
1/5 보강
[INTRODUCTION] The aim of this study was to evaluate the characteristics of bone marrow (BM) basophils in myeloproliferative neoplasm (MPN) using flow cytometry (FCM).
APA
Meng Y, Liao H, et al. (2025). Bone Marrow Basophil Evaluation in Myeloproliferative Neoplasms Using Flow Cytometry.. International journal of laboratory hematology, 47(6), 1074-1082. https://doi.org/10.1111/ijlh.14520
MLA
Meng Y, et al.. "Bone Marrow Basophil Evaluation in Myeloproliferative Neoplasms Using Flow Cytometry.." International journal of laboratory hematology, vol. 47, no. 6, 2025, pp. 1074-1082.
PMID
40615229 ↗
Abstract 한글 요약
[INTRODUCTION] The aim of this study was to evaluate the characteristics of bone marrow (BM) basophils in myeloproliferative neoplasm (MPN) using flow cytometry (FCM). Basophils have been known to be elevated in chronic myeloid leukemia (CML) through blood analyzers or microscopes, but these methods are not reliable for identifying BM basophils, and the changes in BM basophils in non-CML MPN were unclear.
[METHODS] BM basophils of 101 MPN were evaluated by FCM, assessing indices related to basophil levels and CD63/CD203c expression. PB basophils were counted by blood analyzer. The basophil characteristics of MPN were compared to that of 95 individuals without hematopoietic disorders and 30 newly diagnosed myelodysplastic neoplasms (MDS).
[RESULTS] MPN patients showed increased levels of BM basophils and basophils with CD203c expression compared to normal or reactive group. Although weak correlation was observed, BM basophil levels were statistically different from those in PB. Comparing basophil characteristics between non-CML MPN and normal or reactive group, BM basophil indices demonstrated higher elevated counts than PB basophil indices in MPN. BM baso/lym ratio and basophil percentage were most effective in distinguishing non-CML MPN from non-hematopoietic disorders, while PB basophil indices demonstrated limited discriminative value. Most of the basophil indices of CML were different from those of normal group, reactive basophilia, non-CML MPN and MDS. CML could be discriminated from non-hematopoietic disorders by all basophil indices, with BM baso/lym ratio, PB basophil count and PB baso/lym ratio demonstrating superior capacity of discrimination.
[CONCLUSIONS] BM basophil indices, particularly the baso/lym ratio, enhance MPN identification and outperform PB basophil indices in discriminating non-CML MPN from non-hematopoietic disorders. These findings indicate BM basophil analysis by FCM could serve as a potential tool for MPN screening.
[METHODS] BM basophils of 101 MPN were evaluated by FCM, assessing indices related to basophil levels and CD63/CD203c expression. PB basophils were counted by blood analyzer. The basophil characteristics of MPN were compared to that of 95 individuals without hematopoietic disorders and 30 newly diagnosed myelodysplastic neoplasms (MDS).
[RESULTS] MPN patients showed increased levels of BM basophils and basophils with CD203c expression compared to normal or reactive group. Although weak correlation was observed, BM basophil levels were statistically different from those in PB. Comparing basophil characteristics between non-CML MPN and normal or reactive group, BM basophil indices demonstrated higher elevated counts than PB basophil indices in MPN. BM baso/lym ratio and basophil percentage were most effective in distinguishing non-CML MPN from non-hematopoietic disorders, while PB basophil indices demonstrated limited discriminative value. Most of the basophil indices of CML were different from those of normal group, reactive basophilia, non-CML MPN and MDS. CML could be discriminated from non-hematopoietic disorders by all basophil indices, with BM baso/lym ratio, PB basophil count and PB baso/lym ratio demonstrating superior capacity of discrimination.
[CONCLUSIONS] BM basophil indices, particularly the baso/lym ratio, enhance MPN identification and outperform PB basophil indices in discriminating non-CML MPN from non-hematopoietic disorders. These findings indicate BM basophil analysis by FCM could serve as a potential tool for MPN screening.
🏷️ 키워드 / MeSH 📖 같은 키워드 OA만
같은 제1저자의 인용 많은 논문 (5)
- Goosecoid facilitates the metastasis of pancreatic adenocarcinoma by enhancing EMT and stemness via regulating TGF-β/SMAD2/3 signaling.
- Membrane protein-focused CRISPR screen identifies ATP2A2 as a druggable transcriptional co-regulator of CCND1 (cyclin D1) in lung adenocarcinoma.
- Impact of Mobilization Facilitated by Wearable Device Enhanced Patient Monitoring/Electrophysiology Pod-Based Feedback on Postoperative Complications Following Colorectal Cancer Surgery: Randomized Controlled Trial.
- Prognostic biomarkers and regulatory mechanisms associated with lysine β-hydroxybutyrylation modification in prostate cancer.
- Targeted Reprogramming of Tumor Cells by Digoxin-Loaded Immunogenic Nanoparticles Enhances Immunity Against Disseminated Tumor Cells.
🏷️ 같은 키워드 · 무료전문 — 이 논문 MeSH/keyword 기반
- A Phase I Study of Hydroxychloroquine and Suba-Itraconazole in Men with Biochemical Relapse of Prostate Cancer (HITMAN-PC): Dose Escalation Results.
- Self-management of male urinary symptoms: qualitative findings from a primary care trial.
- Clinical and Liquid Biomarkers of 20-Year Prostate Cancer Risk in Men Aged 45 to 70 Years.
- Diagnostic accuracy of Ga-PSMA PET/CT versus multiparametric MRI for preoperative pelvic invasion in the patients with prostate cancer.
- Association of patient health education with the postoperative health related quality of life in low- intermediate recurrence risk differentiated thyroid cancer patients.
- Early local immune activation following intra-operative radiotherapy in human breast tissue.