Protocol for assessing antibody-mediated cellular phagocytosis of lymphoma cells by human and murine macrophages in vitro.

Antibody-dependent phagocytosis is an important process of cell engulfment and degradation by macrophages that holds therapeutical importance in the context of chemo-immunotherapy in, for example, lymphoma. Here, we present an in vitro protocol for determining the antibody-dependent phagocytosis of lymphoma cells by different types of macrophages under different treatment conditions. We describe steps for generating mature macrophages from different sources, usage of different clinically established antibodies, and elucidating the driving cell type of the observed changes in phagocytosis. For complete details on the use and execution of this protocol, please refer to Beielstein et al..