Protocol for isolation of lipid droplets and mitochondria from acute myeloid leukemia cells for lipidomic analysis.
Lipid droplets (LDs) are dynamic organelles critical for lipid and energy homeostasis.
APA
Zhao R, Zhang L, et al. (2025). Protocol for isolation of lipid droplets and mitochondria from acute myeloid leukemia cells for lipidomic analysis.. STAR protocols, 6(4), 104257. https://doi.org/10.1016/j.xpro.2025.104257
MLA
Zhao R, et al.. "Protocol for isolation of lipid droplets and mitochondria from acute myeloid leukemia cells for lipidomic analysis.." STAR protocols, vol. 6, no. 4, 2025, pp. 104257.
PMID
41420867
Abstract
Lipid droplets (LDs) are dynamic organelles critical for lipid and energy homeostasis. LD dysregulation is implicated in various diseases, including acute myeloid leukemia (AML), which exhibits aberrant lipid metabolism and chemoresistance. Here, we describe an effective protocol for the co-isolation of LDs and mitochondria from lipid-deficient cell types, e.g., AML cells. We detail procedures for the isolation, quality verification, and results from mass spectrometry-based lipidomic analysis. This protocol enables the functional study of organellar lipidomic regulation in AML.
MeSH Terms
Lipidomics; Mitochondria; Humans; Leukemia, Myeloid, Acute; Lipid Droplets; Lipid Metabolism; Mass Spectrometry; Cell Fractionation; Cell Line, Tumor
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