Role of Specific miRNA Expression and Nuclear Kappa B Gene Polymorphism as Potential Diagnostic Markers for Chronic Myeloid Leukemia.
[OBJECTIVE] This study assesses microRNA-21 (miR-21) and microRNA-302 (miR-302) levels and examines their association with a nuclear factor kappa-light-chain enhancer of activated B cells (NF-κB) gene
- p-value p < 0.0001
APA
Alhmoud JF, Alqaraleh M, et al. (2026). Role of Specific miRNA Expression and Nuclear Kappa B Gene Polymorphism as Potential Diagnostic Markers for Chronic Myeloid Leukemia.. Medical principles and practice : international journal of the Kuwait University, Health Science Centre, 1-12. https://doi.org/10.1159/000550722
MLA
Alhmoud JF, et al.. "Role of Specific miRNA Expression and Nuclear Kappa B Gene Polymorphism as Potential Diagnostic Markers for Chronic Myeloid Leukemia.." Medical principles and practice : international journal of the Kuwait University, Health Science Centre, 2026, pp. 1-12.
PMID
41615870
Abstract
[OBJECTIVE] This study assesses microRNA-21 (miR-21) and microRNA-302 (miR-302) levels and examines their association with a nuclear factor kappa-light-chain enhancer of activated B cells (NF-κB) gene single nucleotide polymorphism (SNP) in chronic myeloid leukemia (CML) patients, aiming to identify these miRNAs as potential diagnostic biomarkers. The findings could contribute to improved diagnostic and treatment approaches.
[SUBJECT AND METHODS] This study involved 65 patients with CML at Al-Basheer Hospital, Amman, Jordan, and 30 healthy controls. Hematological parameters were analyzed via complete blood count. Gene expression was assessed by real-time PCR to analyze miRNA and NF-KB SNPs in patients' plasma.
[RESULTS] A highly significant difference (p < 0.0001) was observed, indicating that miR-21 expression was higher in CML patients compared to the controls. miR-302 levels were lower in CML patients. The NF-κB Del/Del genotype was associated with a higher white blood cell count compared to the Ins/Ins and Ins/Del genotypes. Platelet counts varied among the CML patients with three polymorphisms.
[CONCLUSION] miR-21 is elevated in CML patients, suggesting an oncogenic role. Our results suggest that miR-302 may serve as a prognostic and diagnostic biomarker. The NF-κB1 gene rs28362491 Del/Del genotype may be associated with an increased risk of CML. Identification of these biomarkers can contribute significantly to improve both diagnosis and treatment strategies.
[SUBJECT AND METHODS] This study involved 65 patients with CML at Al-Basheer Hospital, Amman, Jordan, and 30 healthy controls. Hematological parameters were analyzed via complete blood count. Gene expression was assessed by real-time PCR to analyze miRNA and NF-KB SNPs in patients' plasma.
[RESULTS] A highly significant difference (p < 0.0001) was observed, indicating that miR-21 expression was higher in CML patients compared to the controls. miR-302 levels were lower in CML patients. The NF-κB Del/Del genotype was associated with a higher white blood cell count compared to the Ins/Ins and Ins/Del genotypes. Platelet counts varied among the CML patients with three polymorphisms.
[CONCLUSION] miR-21 is elevated in CML patients, suggesting an oncogenic role. Our results suggest that miR-302 may serve as a prognostic and diagnostic biomarker. The NF-κB1 gene rs28362491 Del/Del genotype may be associated with an increased risk of CML. Identification of these biomarkers can contribute significantly to improve both diagnosis and treatment strategies.