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Whole Genome and Single-Cell RNA Sequencing Reveals Clonal Evolution and Heterogeneity of Secondary Plasma Cell Leukemia: A Case Report.

EJHaem 2026 Vol.7(1) p. e70215

Suzuki T, Yokomori R, Sanda T, Ri M, Iida S

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Secondary plasma cell leukemia (sPCL) is a rare, aggressive manifestation of multiple myeloma (MM).

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APA Suzuki T, Yokomori R, et al. (2026). Whole Genome and Single-Cell RNA Sequencing Reveals Clonal Evolution and Heterogeneity of Secondary Plasma Cell Leukemia: A Case Report.. EJHaem, 7(1), e70215. https://doi.org/10.1002/jha2.70215
MLA Suzuki T, et al.. "Whole Genome and Single-Cell RNA Sequencing Reveals Clonal Evolution and Heterogeneity of Secondary Plasma Cell Leukemia: A Case Report.." EJHaem, vol. 7, no. 1, 2026, pp. e70215.
PMID 41542018
DOI 10.1002/jha2.70215

Abstract

Secondary plasma cell leukemia (sPCL) is a rare, aggressive manifestation of multiple myeloma (MM). We report a 75-year-old Japanese man with anemia as the chief complaint and IgG-λ MM that rapidly progressed to sPCL. Whole genome sequencing using Canopy revealed a major clone with a monoallelic mutation. During progression, a subclone with a biallelic mutation expanded, and the 1q21 copy number increased. Single-cell RNA-sequencing identified an emergent PCL population with high CKS1B expression. These data demonstrate genomic instability and clonal evolution during sPCL development, underscoring the need for approaches accounting for tumor heterogeneity in MM. : The authors have confirmed clinical trial registration is not needed for this submission.

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