Cytogenomic Abnormalities in Children With Acute Lymphoblastic Leukemia From Western Mexico: A Single-Center Fluorescence In Situ Hybridization-Based Study.
[INTRODUCTION] In Mexico, the 5-year overall survival (OS) rate for pediatric acute lymphoblastic leukemia (ALL) ranges from 45% to 85%, markedly lower than the ∼90% reported in high-income countries,
APA
Arreola RMG, Torres MTM, et al. (2026). Cytogenomic Abnormalities in Children With Acute Lymphoblastic Leukemia From Western Mexico: A Single-Center Fluorescence In Situ Hybridization-Based Study.. EJHaem, 7(1), e70220. https://doi.org/10.1002/jha2.70220
MLA
Arreola RMG, et al.. "Cytogenomic Abnormalities in Children With Acute Lymphoblastic Leukemia From Western Mexico: A Single-Center Fluorescence In Situ Hybridization-Based Study.." EJHaem, vol. 7, no. 1, 2026, pp. e70220.
PMID
41561684
Abstract
[INTRODUCTION] In Mexico, the 5-year overall survival (OS) rate for pediatric acute lymphoblastic leukemia (ALL) ranges from 45% to 85%, markedly lower than the ∼90% reported in high-income countries, where cytogenomic testing is essential for accurate risk stratification and therapeutic decision-making. The few available data for Mexican cohorts derive from studies conducted in Mexico City using conventional karyotyping, DNA index analysis, and RT-PCR targeting only four gene fusions. Broader cytogenomic characterization is needed to identify additional prognostic alterations.
[METHODS] We analyzed 170 pediatric ALL cases (150 B-Cell lineage, 10 T-Cell lineage, and 10 mixed phenotype) using fluorescence in situ hybridization (FISH) with a panel of 11 probe sets targeting recurrent cytogenomic abnormalities. All patients were treated according to the Total XV protocol.
[RESULTS] Among 150 B-Cell ALL cases, recurrent cytogenomic abnormalities included ETV6 ( = 19), ( = 7), ( = 5), ::V ( = 10), ::V ( = 7), V:: ( = 11), iAMP21 ( = 8), and deletions involving ( = 38), ( = 7), (8), ( = 1), and ( = 15). Hypodiploidy ( = 2), high-hyperdiploidy ( = 38), low-hyperdiploidy ( = 16), and 1q gain ( = 14) were also identified.
[CONCLUSIONS] Our findings reveal a cytogenomic landscape characterized by a predominance of high-risk abnormalities such as iAMP21 and ::V, together with a lower frequency of low-risk alterations like . The frequent coexistence of secondary abnormalities further supports the relevance of comprehensive cytogenomic profiling for accurate risk assessment. The high diagnostic coverage and rapid turnaround of the FISH-based approach underscore its value as a reliable and efficient diagnostic tool in newly diagnosed ALL.
[TRIAL REGISTRATION] The authors have confirmed clinical trial registration is not needed for this submission.
[METHODS] We analyzed 170 pediatric ALL cases (150 B-Cell lineage, 10 T-Cell lineage, and 10 mixed phenotype) using fluorescence in situ hybridization (FISH) with a panel of 11 probe sets targeting recurrent cytogenomic abnormalities. All patients were treated according to the Total XV protocol.
[RESULTS] Among 150 B-Cell ALL cases, recurrent cytogenomic abnormalities included ETV6 ( = 19), ( = 7), ( = 5), ::V ( = 10), ::V ( = 7), V:: ( = 11), iAMP21 ( = 8), and deletions involving ( = 38), ( = 7), (8), ( = 1), and ( = 15). Hypodiploidy ( = 2), high-hyperdiploidy ( = 38), low-hyperdiploidy ( = 16), and 1q gain ( = 14) were also identified.
[CONCLUSIONS] Our findings reveal a cytogenomic landscape characterized by a predominance of high-risk abnormalities such as iAMP21 and ::V, together with a lower frequency of low-risk alterations like . The frequent coexistence of secondary abnormalities further supports the relevance of comprehensive cytogenomic profiling for accurate risk assessment. The high diagnostic coverage and rapid turnaround of the FISH-based approach underscore its value as a reliable and efficient diagnostic tool in newly diagnosed ALL.
[TRIAL REGISTRATION] The authors have confirmed clinical trial registration is not needed for this submission.