[LncRNA Regulates the Signaling Axis to Affect Proliferation and Apoptosis of Diffuse Large B-Cell Lymphoma Cells].
1/5 보강
[OBJECTIVE] To investigate the effect of long non-coding RNA (lncRNA) on proliferation and apoptosis of diffuse large B-cell lymphoma (DLBCL) cells and the underlying mechanisms.
APA
Xu XW, Adina W, et al. (2026). [LncRNA Regulates the Signaling Axis to Affect Proliferation and Apoptosis of Diffuse Large B-Cell Lymphoma Cells].. Zhongguo shi yan xue ye xue za zhi, 34(1), 45-52. https://doi.org/10.19746/j.cnki.issn.1009-2137.2026.01.007
MLA
Xu XW, et al.. "[LncRNA Regulates the Signaling Axis to Affect Proliferation and Apoptosis of Diffuse Large B-Cell Lymphoma Cells].." Zhongguo shi yan xue ye xue za zhi, vol. 34, no. 1, 2026, pp. 45-52.
PMID
41846336
Abstract
[OBJECTIVE] To investigate the effect of long non-coding RNA (lncRNA) on proliferation and apoptosis of diffuse large B-cell lymphoma (DLBCL) cells and the underlying mechanisms.
[METHODS] qRT-PCR was used to detect the expression of in human B lymphoblastic cells IM-9 and DLBCL cells OCI-Ly1, OCI-Ly3, OCI-Ly4, and OCI-Ly10. OCI-Ly1 cells were divided into four groups: si-NC (negative control), si-, si-+ inhibitor, and si-+ inhibitor+si-. OCI-Ly3 cells were divided into four groups: Control, , + mimic, and + mimic+. Dual-luciferase reporter assay was performed to verify the targeting relationship between and , as well as the targeting relationship between and . CCK-8 assay was used to detect cell proliferative capacity. Flow cytometry was used to detect cell apoptosis, and Western blot was used to detect the expression of HMGA2 protein.
[RESULTS] The expression of in OCI-Ly1, OCI-Ly3, OCI-Ly4, and OCI-Ly10 cells was significantly higher than that in IM-9 cells. Dual-luciferase reporter assay confirmed that could bind specifically to , and is a target of . In OCI-Ly1 cells, the si- group exhibited significantly reduced cell proliferation and HMGA2 protein expression ( <0.05) and markedly increased apoptosis ( <0.001) compared with the si-NC group; The si- + inhibitor group exhibited significantly higher proliferation ( <0.01) and substantially lower apoptosis ( <0.001) than the si- group; Furthermore, the si- + inhibitor + si- group displayed significantly decreased proliferation ( <0.01) and dramatically elevated apoptosis ( <0.001) when compared with the si- + inhibitor group. In OCI-Ly3 cells, the group displayed significantly enhanced proliferation and elevated HMGA2 protein expression ( <0.05) with markedly reduced apoptosis ( <0.001) compared to the Control group. Conversely, the + mimic group showed dramatically impaired proliferation ( <0.001) and substantially increased apoptosis ( < 0.001) versus the group. Notably, the + mimic + group rescued proliferation ( <0.05) and suppressed apoptosis ( <0.001) relative to the + mimic group.
[CONCLUSION] promotes the proliferation and inhibits the apoptosis of DLBCL cells by regulating the signaling axis.
[METHODS] qRT-PCR was used to detect the expression of in human B lymphoblastic cells IM-9 and DLBCL cells OCI-Ly1, OCI-Ly3, OCI-Ly4, and OCI-Ly10. OCI-Ly1 cells were divided into four groups: si-NC (negative control), si-, si-+ inhibitor, and si-+ inhibitor+si-. OCI-Ly3 cells were divided into four groups: Control, , + mimic, and + mimic+. Dual-luciferase reporter assay was performed to verify the targeting relationship between and , as well as the targeting relationship between and . CCK-8 assay was used to detect cell proliferative capacity. Flow cytometry was used to detect cell apoptosis, and Western blot was used to detect the expression of HMGA2 protein.
[RESULTS] The expression of in OCI-Ly1, OCI-Ly3, OCI-Ly4, and OCI-Ly10 cells was significantly higher than that in IM-9 cells. Dual-luciferase reporter assay confirmed that could bind specifically to , and is a target of . In OCI-Ly1 cells, the si- group exhibited significantly reduced cell proliferation and HMGA2 protein expression ( <0.05) and markedly increased apoptosis ( <0.001) compared with the si-NC group; The si- + inhibitor group exhibited significantly higher proliferation ( <0.01) and substantially lower apoptosis ( <0.001) than the si- group; Furthermore, the si- + inhibitor + si- group displayed significantly decreased proliferation ( <0.01) and dramatically elevated apoptosis ( <0.001) when compared with the si- + inhibitor group. In OCI-Ly3 cells, the group displayed significantly enhanced proliferation and elevated HMGA2 protein expression ( <0.05) with markedly reduced apoptosis ( <0.001) compared to the Control group. Conversely, the + mimic group showed dramatically impaired proliferation ( <0.001) and substantially increased apoptosis ( < 0.001) versus the group. Notably, the + mimic + group rescued proliferation ( <0.05) and suppressed apoptosis ( <0.001) relative to the + mimic group.
[CONCLUSION] promotes the proliferation and inhibits the apoptosis of DLBCL cells by regulating the signaling axis.
MeSH Terms
Humans; RNA, Long Noncoding; Apoptosis; MicroRNAs; Cell Proliferation; Lymphoma, Large B-Cell, Diffuse; Signal Transduction; Cell Line, Tumor; HMGA2 Protein