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B-Cell Differentiation of Human Hematopoietic Progenitors Is Efficiently Supported by Wharton Jelly-Derived Mesenchymal Stem Cells.

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European journal of immunology 2026 Vol.56(4) p. e70186 OA Mesenchymal stem cell research
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PubMed DOI PMC OpenAlex 마지막 보강 2026-04-30
OpenAlex 토픽 · Mesenchymal stem cell research Acute Lymphoblastic Leukemia research Hematopoietic Stem Cell Transplantation

Collet L, Ouled-Haddou H, Ghamlouch H, Darwiche W, Gomila C, Gubler B

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Mesenchymal stem cells (MSC) represent the main stromal component of the bone marrow (BM) niche and are crucial to maintain hematopoietic tissue homeostasis.

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APA Louison Collet, Hakim Ouled‐Haddou, et al. (2026). B-Cell Differentiation of Human Hematopoietic Progenitors Is Efficiently Supported by Wharton Jelly-Derived Mesenchymal Stem Cells.. European journal of immunology, 56(4), e70186. https://doi.org/10.1002/eji.70186
MLA Louison Collet, et al.. "B-Cell Differentiation of Human Hematopoietic Progenitors Is Efficiently Supported by Wharton Jelly-Derived Mesenchymal Stem Cells.." European journal of immunology, vol. 56, no. 4, 2026, pp. e70186.
PMID 41934200 ↗
DOI 10.1002/eji.70186

Abstract

Mesenchymal stem cells (MSC) represent the main stromal component of the bone marrow (BM) niche and are crucial to maintain hematopoietic tissue homeostasis. MSC exhibits extraordinary and multiple properties. In terms of expanding potential and differentiation capacity, Wharton jelly MSC (WJ-MSC), derived from the umbilical cord, was described as being greater and more performing than MSC from BM or other sources. WJ-MSC mimics the hematopoietic niche and supports hematopoietic stem cells (HSC) expansion ex vivo. This study aimed to evaluate the effects of human WJ-MSC cocultured with HSC in a B-cell differentiation protocol. Remarkably, results highlight WJ-MSC use as a preferable feeder layer to efficiently support HSC commitment toward the B-lineage. Over 11 days of HSC coculture with WJ-MSC, B-cell genes (E2A, RAG1, RAG2, etc.) expression patterns and B-cell markers (CD19, immunoglobulin chain, etc.) acquisition were evidenced. WJ-MSc were also able to unlock the B-lineage differentiation blockade of the acute lymphoblastic leukemia cell line Nalm16. This model might provide a new strategy to support ex vivo B-cell differentiation using the powerful properties of WJ-MSC. This study implements a new approach to improve understanding of B-leukemogenesis and B-cell acute lymphoblastic leukemia (B-ALL) pathophysiology.

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