Evaluation of -derived outer membrane vesicles on the expression of inflammatory cytokines.
1/5 보강
[BACKGROUND AND OBJECTIVES] infection has been increasingly linked to extra-gastric diseases.
APA
Mohammadbeigi M, Peymani A, et al. (2025). Evaluation of -derived outer membrane vesicles on the expression of inflammatory cytokines.. Iranian journal of microbiology, 17(6), 966-976. https://doi.org/10.18502/ijm.v17i6.20365
MLA
Mohammadbeigi M, et al.. "Evaluation of -derived outer membrane vesicles on the expression of inflammatory cytokines.." Iranian journal of microbiology, vol. 17, no. 6, 2025, pp. 966-976.
PMID
41510043
Abstract
[BACKGROUND AND OBJECTIVES] infection has been increasingly linked to extra-gastric diseases. Outer membrane vesicles are a key virulence factor of . This study investigates the influence of -derived outer membrane vesicles on inflammatory marker expression in human hepatoma cells (HepG2).
[MATERIALS AND METHODS] Outer membrane vesicles were isolated through ultracentrifugation and characterized using dynamic light scattering technique (DLS) and a Field Emission Scanning Electron Microscope (FE-SEM). Protein concentrations were measured via the Bradford assay. HepG2 cells treated with outer membrane vesicles were analyzed for IL-6, TNF-α, TLR-4, TGF-β, and PPAR-γ mRNA expression by RT-qPCR. Cell viability was assessed through an MTT assay. The prevalence of virulence-associated genes (, , and ) was determined by PCR.
[RESULTS] The results showed a high prevalence of (91.7%), (75%), and (66.7%). FE-SEM and DLS analyses confirmed the presence of bleb-shaped nanovesicles ranging in size from 50 to 450 nm. -derived outer membrane vesicles significantly upregulated the expression of pro-inflammatory markers (TLR-4, PPAR-γ, TNF-α, and IL-6), while downregulating TGF-β expression.
[CONCLUSION] These findings underscore the potential role of nanoparticles in driving inflammatory responses and influencing host cell signaling, which may play a key role in liver-related pathologies.
[MATERIALS AND METHODS] Outer membrane vesicles were isolated through ultracentrifugation and characterized using dynamic light scattering technique (DLS) and a Field Emission Scanning Electron Microscope (FE-SEM). Protein concentrations were measured via the Bradford assay. HepG2 cells treated with outer membrane vesicles were analyzed for IL-6, TNF-α, TLR-4, TGF-β, and PPAR-γ mRNA expression by RT-qPCR. Cell viability was assessed through an MTT assay. The prevalence of virulence-associated genes (, , and ) was determined by PCR.
[RESULTS] The results showed a high prevalence of (91.7%), (75%), and (66.7%). FE-SEM and DLS analyses confirmed the presence of bleb-shaped nanovesicles ranging in size from 50 to 450 nm. -derived outer membrane vesicles significantly upregulated the expression of pro-inflammatory markers (TLR-4, PPAR-γ, TNF-α, and IL-6), while downregulating TGF-β expression.
[CONCLUSION] These findings underscore the potential role of nanoparticles in driving inflammatory responses and influencing host cell signaling, which may play a key role in liver-related pathologies.