Single-Cell sequencing investigation of endoplasmic reticulum stress-related genes in gastric cancer prognostic models and identification of NOX5 as a novel therapeutic target.
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OpenAlex 토픽 ·
Endoplasmic Reticulum Stress and Disease
Ferroptosis and cancer prognosis
Protein Tyrosine Phosphatases
[BACKGROUND] Gastric cancer (GC) has a poor prognosis, and its pathogenesis remains incompletely understood.
- p-value p < 0.01
APA
Yue Fan, feng liu, et al. (2026). Single-Cell sequencing investigation of endoplasmic reticulum stress-related genes in gastric cancer prognostic models and identification of NOX5 as a novel therapeutic target.. Translational oncology, 68, 102780. https://doi.org/10.1016/j.tranon.2026.102780
MLA
Yue Fan, et al.. "Single-Cell sequencing investigation of endoplasmic reticulum stress-related genes in gastric cancer prognostic models and identification of NOX5 as a novel therapeutic target.." Translational oncology, vol. 68, 2026, pp. 102780.
PMID
42013548
Abstract
[BACKGROUND] Gastric cancer (GC) has a poor prognosis, and its pathogenesis remains incompletely understood. Endoplasmic reticulum stress (ERS) may influence GC progression, yet ERS-based prognostic models are lacking. We aimed to develop an ERS-related prognostic signature using single-cell analysis and identify potential therapeutic targets.
[METHODS] Integrated analyses including single-cell RNA sequencing, cell‒cell communication, GSVA enrichment, and drug sensitivity inference were performed. A 14-gene prognostic model was constructed using Cox regression and LASSO, and validated via Kaplan-Meier and ROC curves. NOX5 function was assessed through in vitro proliferation and migration assays.
[RESULTS] The ERS signature outperformed clinicopathological parameters in predicting GC survival, with AUCs of 0.75, 0.71, and 0.64 for 1-, 3-, and 5-year OS, respectively. Patients were stratified into high- and low-risk groups with distinct immune checkpoint profiles. NOX5, the top risk gene (HR > 2.0), was upregulated in GC, and its knockdown significantly suppressed AGS and MKN-45 cell proliferation and migration (p < 0.01).
[CONCLUSION] The ERS-related signature is a promising independent prognostic and predictive biomarker for GC. NOX5 represents a novel potential therapeutic target.
[METHODS] Integrated analyses including single-cell RNA sequencing, cell‒cell communication, GSVA enrichment, and drug sensitivity inference were performed. A 14-gene prognostic model was constructed using Cox regression and LASSO, and validated via Kaplan-Meier and ROC curves. NOX5 function was assessed through in vitro proliferation and migration assays.
[RESULTS] The ERS signature outperformed clinicopathological parameters in predicting GC survival, with AUCs of 0.75, 0.71, and 0.64 for 1-, 3-, and 5-year OS, respectively. Patients were stratified into high- and low-risk groups with distinct immune checkpoint profiles. NOX5, the top risk gene (HR > 2.0), was upregulated in GC, and its knockdown significantly suppressed AGS and MKN-45 cell proliferation and migration (p < 0.01).
[CONCLUSION] The ERS-related signature is a promising independent prognostic and predictive biomarker for GC. NOX5 represents a novel potential therapeutic target.
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