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Fucoxanthin mitigates aflatoxin B1-triggered hepatotoxicity in HepG2 cells via modulation of oxidative stress, inflammatory cytokines, and caspases cascade.

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Ecotoxicology and environmental safety 📖 저널 OA 31.7% 2021: 0/1 OA 2024: 1/3 OA 2025: 0/28 OA 2026: 19/31 OA 2021~2026 2025 Vol.303() p. 118777
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Elmorsy EM, Abdelkader A, Ali NE, Elgendy FS, Elbaghdady HAM, Mohammed LA

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Aflatoxins (AFB1) are harmful secondary metabolites generated by filamentous fungi with a profound hepatotoxic effect.

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APA Elmorsy EM, Abdelkader A, et al. (2025). Fucoxanthin mitigates aflatoxin B1-triggered hepatotoxicity in HepG2 cells via modulation of oxidative stress, inflammatory cytokines, and caspases cascade.. Ecotoxicology and environmental safety, 303, 118777. https://doi.org/10.1016/j.ecoenv.2025.118777
MLA Elmorsy EM, et al.. "Fucoxanthin mitigates aflatoxin B1-triggered hepatotoxicity in HepG2 cells via modulation of oxidative stress, inflammatory cytokines, and caspases cascade.." Ecotoxicology and environmental safety, vol. 303, 2025, pp. 118777.
PMID 40752154 ↗

Abstract

Aflatoxins (AFB1) are harmful secondary metabolites generated by filamentous fungi with a profound hepatotoxic effect. Fucoxanthin (FX) is a flavonoid with a well-known cytoprotective action. Here, we evaluated the ability of FX to mitigate AFB1-triggered hepatotoxicity using the HepG2 cell line. Data revealed that AFB1 was cytotoxic to the hepatic cells in a concentration-dependent manner. AFB1 was shown to alter cytochrome P450 activities and its coding gene expression. It also caused genotoxicity to the liver cells with increased comet tail DNA parameters. Furthermore, AFB1 increased NF-κB/p65 and the proinflammatory cytokines accompanied by inhibition of cellular antioxidants, including CAT, SOD, NRF2, and HO-1 gene expressions, which leads to increased ROS generation and LPO in AFB1-treated cells. Besides, AFB1 increased the release of cytochrome c into the cytoplasm and enhanced the activities of caspases-3, -8, and -9. This was accompanied by an increased Bax/Bcl2 ratio and the activation of apoptosis pathways. FX (2.5-5 µM) was shown to mitigate AFB1-induced cytotoxicity to variable degrees. Molecular docking indicated the toxic effect of AFB1 as well as the preventive action of FX. These data suggest the potential therapeutic anti-inflammatory, antioxidant, and anti-apoptotic benefits of FX in preventing and treating AFB1 hepatotoxicity.

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