CD133-Derived Exosomes Carrying EIF3B Mediate Cell Metasta sis and Stemness in Colorectal Cancer.
[BACKGROUND] Colorectal cancer (CRC) is among the most widespread malignancies worldwide and is a leading cause for cancer mortality.
APA
Liao X, Han X, et al. (2026). CD133-Derived Exosomes Carrying EIF3B Mediate Cell Metasta sis and Stemness in Colorectal Cancer.. Current cancer drug targets, 26(1), 120-127. https://doi.org/10.2174/0115680096346009250628215410
MLA
Liao X, et al.. "CD133-Derived Exosomes Carrying EIF3B Mediate Cell Metasta sis and Stemness in Colorectal Cancer.." Current cancer drug targets, vol. 26, no. 1, 2026, pp. 120-127.
PMID
40660438
Abstract
[BACKGROUND] Colorectal cancer (CRC) is among the most widespread malignancies worldwide and is a leading cause for cancer mortality. The interstitial interaction between cancer and stem cells is important during cancer cell metastasis.
[OBJECTIVE] In this study, we aimed to elucidate the regulatory role and the underlying mechanisms controlling the activity of exosomes derived from cancer stem cells (CSCs).
[METHODS] Our group isolated exosomes from CSCs and non-CSCs to examine their regulatory mechanisms using Transwell migration, Cell Counting Kit-8 (CCK-8), and 5-ethynyl- 2'-deoxyuridine (EdU) assays.
[RESULTS] The role of Eukaryotic Translation Initiation Factor 3 Subunit B (EIF3B) in CRC was examined using an in vivo tumorigenesis mouse model. It was found that treatment with exosomes isolated from CD133 cells (CD133+Exos) promoted the proliferation and migration of SW480 cells. The downregulation of EIF3B reduced the proliferation and migration- promoting effects of CD133+ Exos on SW480 cells. Furthermore, CD133 Exos treatment promoted the tumorigenesis of SW480 cells.
[CONCLUSION] Our findings demonstrate that CSC-derived exosomes transport EIF3B into CRC cells to initiate epithelial-to-mesenchymal transition (EMT) and promote metastasis.
[OBJECTIVE] In this study, we aimed to elucidate the regulatory role and the underlying mechanisms controlling the activity of exosomes derived from cancer stem cells (CSCs).
[METHODS] Our group isolated exosomes from CSCs and non-CSCs to examine their regulatory mechanisms using Transwell migration, Cell Counting Kit-8 (CCK-8), and 5-ethynyl- 2'-deoxyuridine (EdU) assays.
[RESULTS] The role of Eukaryotic Translation Initiation Factor 3 Subunit B (EIF3B) in CRC was examined using an in vivo tumorigenesis mouse model. It was found that treatment with exosomes isolated from CD133 cells (CD133+Exos) promoted the proliferation and migration of SW480 cells. The downregulation of EIF3B reduced the proliferation and migration- promoting effects of CD133+ Exos on SW480 cells. Furthermore, CD133 Exos treatment promoted the tumorigenesis of SW480 cells.
[CONCLUSION] Our findings demonstrate that CSC-derived exosomes transport EIF3B into CRC cells to initiate epithelial-to-mesenchymal transition (EMT) and promote metastasis.
MeSH Terms
Exosomes; Colorectal Neoplasms; Humans; Animals; Eukaryotic Initiation Factor-3; AC133 Antigen; Mice; Neoplastic Stem Cells; Cell Proliferation; Cell Movement; Epithelial-Mesenchymal Transition; Cell Line, Tumor; Mice, Nude; Mice, Inbred BALB C
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