Baihua Dangen Tang inhibits the peritoneal metastasis of colon cancer by modulating the bacterial metabolite gentisic acid to suppress MDSCs.
1/5 보강
[BACKGROUND] The treatment principle of Lingnan traditional compound Baihua Dangen Tang includes 'clearing heat and removing toxins, resolving stasis and dispersing stagnation, and dispelling wind and
APA
Xun J, Han Y, et al. (2026). Baihua Dangen Tang inhibits the peritoneal metastasis of colon cancer by modulating the bacterial metabolite gentisic acid to suppress MDSCs.. Phytomedicine : international journal of phytotherapy and phytopharmacology, 150, 157720. https://doi.org/10.1016/j.phymed.2025.157720
MLA
Xun J, et al.. "Baihua Dangen Tang inhibits the peritoneal metastasis of colon cancer by modulating the bacterial metabolite gentisic acid to suppress MDSCs.." Phytomedicine : international journal of phytotherapy and phytopharmacology, vol. 150, 2026, pp. 157720.
PMID
41422728
Abstract
[BACKGROUND] The treatment principle of Lingnan traditional compound Baihua Dangen Tang includes 'clearing heat and removing toxins, resolving stasis and dispersing stagnation, and dispelling wind and removing dampness', which is compatible with the pathogenesis of peritoneal cancer, which is characterized by 'dampness, heat, stasis and toxin'. Therefore, Baihua Dangen Tang may be an effective treatment for peritoneal metastasis of colon cancer.
[OBJECTIVE] To systematically elucidate the effects and mechanisms of Baihua Dangen Tang in inhibiting the peritoneal metastasis of colon cancer.
[METHODS] In this study, a peritoneal metastasis model of colon cancer was constructed by intraperitoneal injection of CT26/MC38 cells (1 × 10) for 14 days. Abdominal circumference, ascites volume, tumor weight and Ki67 expression were analyzed. Mass spectrometry and flow cytometry were used to analyze changes in immune cells, such as MDSCs and T-cell subsets, in ascites and peripheral blood. Changes in the fecal flora and metabolites were analyzed by macrogenomic and untargeted metabolomics to screen for key differential flora metabolites. In vitro experiments were performed to determine the effects of Baihua Dangen Tang and differential flora metabolites on tumor cell migration and invasion and MDSC function.
[RESULTS] In this study, we observed that Baihua Dangen Tang significantly inhibited peritoneal metastasis of colon cancer; reduced abdominal circumference, bloody ascites content, and metastatic tumor weight; and decreased metastatic tumor Ki67 expression. Analysis of ascites and peripheral blood by mass spectrometry and flow cytometry revealed that Baihua Dangen Tang increased the numbers of CD4 T cells and CD8 T cells and decreased the number of MDSCs in ascites and peripheral blood. Combined macrogenomic and metabolomic analyses revealed that the metabolite gentisic acid, which was positively correlated with Lactobacillus spp. and negatively correlated with Desulfovibrio spp., significantly changed. In vivo experiments confirmed that gentisic acid inhibited the progression of CT26 peritoneal metastases. In vitro experiments confirmed that Baihua Dangen Tang and its regulatory bacterial colony metabolite gentisic acid inhibited the migration and invasion ability of colon cancer cells and their recruitment of MDSCs, in addition to directly inhibiting the function of MDSCs, thus enhancing T-cell activity.
[CONCLUSION] Baihua Dangen Tang and its microbiota-derived metabolite gentisic acid inhibit cancer cell metastasis and MDSCs function, demonstrating multitarget suppression of colorectal cancer peritoneal metastasis.
[OBJECTIVE] To systematically elucidate the effects and mechanisms of Baihua Dangen Tang in inhibiting the peritoneal metastasis of colon cancer.
[METHODS] In this study, a peritoneal metastasis model of colon cancer was constructed by intraperitoneal injection of CT26/MC38 cells (1 × 10) for 14 days. Abdominal circumference, ascites volume, tumor weight and Ki67 expression were analyzed. Mass spectrometry and flow cytometry were used to analyze changes in immune cells, such as MDSCs and T-cell subsets, in ascites and peripheral blood. Changes in the fecal flora and metabolites were analyzed by macrogenomic and untargeted metabolomics to screen for key differential flora metabolites. In vitro experiments were performed to determine the effects of Baihua Dangen Tang and differential flora metabolites on tumor cell migration and invasion and MDSC function.
[RESULTS] In this study, we observed that Baihua Dangen Tang significantly inhibited peritoneal metastasis of colon cancer; reduced abdominal circumference, bloody ascites content, and metastatic tumor weight; and decreased metastatic tumor Ki67 expression. Analysis of ascites and peripheral blood by mass spectrometry and flow cytometry revealed that Baihua Dangen Tang increased the numbers of CD4 T cells and CD8 T cells and decreased the number of MDSCs in ascites and peripheral blood. Combined macrogenomic and metabolomic analyses revealed that the metabolite gentisic acid, which was positively correlated with Lactobacillus spp. and negatively correlated with Desulfovibrio spp., significantly changed. In vivo experiments confirmed that gentisic acid inhibited the progression of CT26 peritoneal metastases. In vitro experiments confirmed that Baihua Dangen Tang and its regulatory bacterial colony metabolite gentisic acid inhibited the migration and invasion ability of colon cancer cells and their recruitment of MDSCs, in addition to directly inhibiting the function of MDSCs, thus enhancing T-cell activity.
[CONCLUSION] Baihua Dangen Tang and its microbiota-derived metabolite gentisic acid inhibit cancer cell metastasis and MDSCs function, demonstrating multitarget suppression of colorectal cancer peritoneal metastasis.