Simultaneous Study of Circular RNAs and Messenger RNAs in Colorectal Cancer: The Unbalanced Fate of a Couple?
[BACKGROUND/OBJECTIVES] Circular RNAs (circRNAs) are emerging players in human diseases, with functions as part of competing endogenous networks.
APA
Levacher C, Delfosse J, et al. (2026). Simultaneous Study of Circular RNAs and Messenger RNAs in Colorectal Cancer: The Unbalanced Fate of a Couple?. Cancers, 18(3). https://doi.org/10.3390/cancers18030496
MLA
Levacher C, et al.. "Simultaneous Study of Circular RNAs and Messenger RNAs in Colorectal Cancer: The Unbalanced Fate of a Couple?." Cancers, vol. 18, no. 3, 2026.
PMID
41681969
Abstract
[BACKGROUND/OBJECTIVES] Circular RNAs (circRNAs) are emerging players in human diseases, with functions as part of competing endogenous networks. Given the importance of messenger RNA (mRNA) regulation in human diseases and the potential of circRNAs in this regulation, we studied the circRNA-mRNA couple in blood within a cohort of 712 patients suspected of having hereditary colorectal cancer (CRC) and 249 matched controls.
[METHODS] The circRNA-mRNA couple was studied by SEALigHTS (Splice and Expression Analyses by exon Ligation and High-Throughput Sequencing) with a panel of 23 genes involved in CRC predisposition, comprising 788 probes designed at exon ends, enabling the exploration of all exon-exon junctions. Following reverse transcription and probe hybridization on cDNA, nearby probes were ligated, and the number of ligations was quantified using unique molecular identifiers and sequencing.
[RESULTS] We identified 220 circular junctions, including 47 novel ones. The circRNA/mRNA ratio was 2.42-fold higher in patients compared to controls ( < 10), irrespective of age at cancer onset. This increase was mainly driven by (fold change 3.84) and a single circPOLD1(3,2) with oncogenic potential Conclusions: This study supports the existence of a physiological balance between circRNA and mRNA that can be disrupted under pathological conditions. It rules out a competitive mechanism between circular and linear transcripts in CRC predisposition and raises questions about the role of specific circRNAs in the development of CRC, either as a cause or a consequence.
[METHODS] The circRNA-mRNA couple was studied by SEALigHTS (Splice and Expression Analyses by exon Ligation and High-Throughput Sequencing) with a panel of 23 genes involved in CRC predisposition, comprising 788 probes designed at exon ends, enabling the exploration of all exon-exon junctions. Following reverse transcription and probe hybridization on cDNA, nearby probes were ligated, and the number of ligations was quantified using unique molecular identifiers and sequencing.
[RESULTS] We identified 220 circular junctions, including 47 novel ones. The circRNA/mRNA ratio was 2.42-fold higher in patients compared to controls ( < 10), irrespective of age at cancer onset. This increase was mainly driven by (fold change 3.84) and a single circPOLD1(3,2) with oncogenic potential Conclusions: This study supports the existence of a physiological balance between circRNA and mRNA that can be disrupted under pathological conditions. It rules out a competitive mechanism between circular and linear transcripts in CRC predisposition and raises questions about the role of specific circRNAs in the development of CRC, either as a cause or a consequence.