N-aminophthalimide-derived unsaturated PC reaction-assisted analysis of carbon-carbon double bond position and cis/trans isomerism in fatty acid chains based on UHPLC-ESI-MS/MS.
기술보고
2/5 보강
OpenAlex 토픽 ·
Metabolomics and Mass Spectrometry Studies
Molecular Sensors and Ion Detection
Peroxisome Proliferator-Activated Receptors
[BACKGROUND] Phosphatidylcholines (PCs) are key components of biological membranes and play crucial roles in key physiological processes, where the positions and cis/trans isomerism of carbon-carbon d
APA
Chunlin Yue, Lingyu Zhao, et al. (2026). N-aminophthalimide-derived unsaturated PC reaction-assisted analysis of carbon-carbon double bond position and cis/trans isomerism in fatty acid chains based on UHPLC-ESI-MS/MS.. Analytica chimica acta, 1401, 345374. https://doi.org/10.1016/j.aca.2026.345374
MLA
Chunlin Yue, et al.. "N-aminophthalimide-derived unsaturated PC reaction-assisted analysis of carbon-carbon double bond position and cis/trans isomerism in fatty acid chains based on UHPLC-ESI-MS/MS.." Analytica chimica acta, vol. 1401, 2026, pp. 345374.
PMID
41903979 ↗
Abstract 한글 요약
[BACKGROUND] Phosphatidylcholines (PCs) are key components of biological membranes and play crucial roles in key physiological processes, where the positions and cis/trans isomerism of carbon-carbon double bonds (CC) in fatty acyl chains critically influence physiological functions and disease mechanisms. The conventional LC-MS technique cannot simultaneously resolve the positional and cis/trans isomers of phosphatidylcholine.
[RESULT] To address the limitations of conventional LC-MS/MS in distinguishing PC isomers, we developed a novel method combining N-aminophthalimide (PhthNH) derivatization with UHPLC-ESI-MS/MS. This approach leverages the specific reaction between PhthNH and CC bonds to form N-aziridine adducts, enabling precise localization of double bond positions through characteristic fragment ions while achieving baseline chromatographic separation of cis/trans isomers in monounsaturated PC.
[SIGNIFICANCE] A derivatization workflow and data analysis procedure for simultaneously detecting PC CC bonds positional and cis/tans isomers was successfully established and optimized. The method successfully identified 31CC positional isomers in plasma samples and revealed significant differences in PC isomer abundance between colorectal cancer patients and healthy controls, establishing a powerful new tool for investigating PC biology and disease associations.
[RESULT] To address the limitations of conventional LC-MS/MS in distinguishing PC isomers, we developed a novel method combining N-aminophthalimide (PhthNH) derivatization with UHPLC-ESI-MS/MS. This approach leverages the specific reaction between PhthNH and CC bonds to form N-aziridine adducts, enabling precise localization of double bond positions through characteristic fragment ions while achieving baseline chromatographic separation of cis/trans isomers in monounsaturated PC.
[SIGNIFICANCE] A derivatization workflow and data analysis procedure for simultaneously detecting PC CC bonds positional and cis/tans isomers was successfully established and optimized. The method successfully identified 31CC positional isomers in plasma samples and revealed significant differences in PC isomer abundance between colorectal cancer patients and healthy controls, establishing a powerful new tool for investigating PC biology and disease associations.
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