Transcriptionally Up-regulates Expression to Mediate Cell Proliferation in Non-small-cell Lung Cancer Cells.

[BACKGROUND/AIM] The AXL receptor tyrosine kinase (AXL) has been identified as a key driver of tumor progression and chemoresistance in non-small-cell lung cancer (NSCLC). However, the molecular mechanisms underlying AXL-mediated oncogenesis remain unclear. This study aimed to identify novel AXL downstream genes involved in NSCLC progression.

[MATERIALS AND METHODS] Transcriptomic RNA sequencing and analysis were performed to identify genes downstream from . Cellular proliferation was assessed using the CCK-8 assay. Glucose uptake was measured using a glucose uptake assay. mRNA expression levels of interferon-stimulated gene 15 () were analyzed reverse transcription-quantitative polymerase chain reaction, and the regulation of transcription by AXL was evaluated through an promoter activity assay.

[RESULTS] Transcriptomic RNA sequencing revealed as a novel downstream target of expression significantly enhanced cellular proliferation and glucose uptake in NSCLC cells. transcriptionally upregulated expression by modulating its 5'-promoter activity.

[CONCLUSION] is a newly identified target of and may serve as a potential therapeutic target to overcome resistance in patients with NSCLC receiving AXL-targeted therapies.