Smartphone-assisted colorimetric detection of FEN1 and its inhibitors via RCA-magnetic beads-urease cascade amplification.
1/5 보강
Given the pivotal role of Flap endonuclease 1 (FEN1) in tumor pathogenesis and progression, the advancement of its activity and inhibitor assays holds significant importance for cancer research and dr
APA
Talap J, Tang D, et al. (2025). Smartphone-assisted colorimetric detection of FEN1 and its inhibitors via RCA-magnetic beads-urease cascade amplification.. Biosensors & bioelectronics, 290, 117969. https://doi.org/10.1016/j.bios.2025.117969
MLA
Talap J, et al.. "Smartphone-assisted colorimetric detection of FEN1 and its inhibitors via RCA-magnetic beads-urease cascade amplification.." Biosensors & bioelectronics, vol. 290, 2025, pp. 117969.
PMID
40929784 ↗
Abstract 한글 요약
Given the pivotal role of Flap endonuclease 1 (FEN1) in tumor pathogenesis and progression, the advancement of its activity and inhibitor assays holds significant importance for cancer research and drug screening. Herein, we proposed a convenient, visual and sensitive colorimetric biosensing platform for FEN1 activity detection by integrating the robust signal amplification power of rolling circle amplification (RCA), the target enrichment capability of magnetic beads (MB), and the high efficiency and visualization of urease-mediated litmus test. Based on the significant color transition with a clear response mechanism, quantitative analysis can be achieved by either spectroscopic or smartphone-based detection. The smartphone-assisted approach demonstrated excellent FEN1 activity detection performance within the range of 2 × 10 U/μL to 0.02 U/μL, with a detection limit of 1.5 × 10 U/μL. The practical utility of this method was validated by assessing differential FEN1 expression profiles in both cytoplasmic and nuclear protein extracts of lung cancer cells versus bronchial epithelial cells. Furthermore, experimental verification confirmed that our urease-mediated system demonstrates enhanced signal-to-noise ratio due to its insusceptibility to the peroxidase-like activity of FeO in MB, while sequence modification enables this platform to achieve broad applicability for detecting other targets.
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